Biomimetic synthesis of silver nanoparticles from Streptomyces atrovirens and their potential anticancer activity against human breast cancer cells. Issue 8 (11th September 2017)

• Record Type: Journal Article
• Authors: Subbaiya, Ramasamy; Saravanan, Muthupandian; Priya, Andavar Raja; Shankar, Konathala Ravi; Selvam, Masilamani; Ovais, Muhammad; Balajee, Ramachandran; Barabadi, Hamed
• Is Part Of: IET nanobiotechnology. Volume 11:Issue 8(2017)
• Page Start: 965
• Page End: 972
• Publication Date: 2017-09-11
• Subjects: silver -- nanoparticles -- nanomedicine -- antibacterial activity -- biomedical materials -- tumours -- cancer -- toxicology -- nanofabrication -- microorganisms -- reduction (chemical) -- ultraviolet spectra -- visible spectra -- atomic force microscopy -- transmission electron microscopy -- Fourier transform infrared spectra -- X‐ray diffraction -- biomimetics; acridine orange -- ethidium bromide double staining methods -- cell morphological analysis -- alternative biomaterials -- human breast cancer therapy -- time 16 s -- Ag -- dose dependence -- MCF‐7 breast cancer cell line inhibition -- cancer cell growth inhibition -- 3‐(4, 5‐dimethylthiazol‐2‐yl)‐2, 5‐diphenyltetrazolium bromide assay -- X‐ray diffraction -- Fourier transform infrared spectroscopy -- transmission electron microscopy -- atomic force microscopy -- ultraviolet‐visible spectrophotometer -- bioanalytical techniques -- ribotyping method -- isolated strain -- marine soil sample -- bioreduction method -- cell death -- Michigan cancer foundation‐7 breast cancer cells -- cytotoxic effects -- antitumour potential -- antimicrobial activity -- human breast cancer cells -- potential anticancer activity -- Streptomyces atrovirens -- silver nanoparticles -- biomimetic synthesis; Biotechnology -- Periodicals; Nanotechnology -- Periodicals; 660.6
• DOI: 10.1049/iet-nbt.2016.0222
• Languages: English
• ISSNs: 1751-8741

Abstract

Abstract : Silver nanoparticles (AgNPs) have been undeniable for its antimicrobial activity while its antitumour potential is still limited. Therefore, the present study focused on determining cytotoxic effects of AgNPs on Michigan cancer foundation‐7 (MCF‐7) breast cancer cells and its corresponding mechanism of cell death. Herein, the authors developed a bio‐reduction method for AgNPs synthesis using actinomycetes isolated from marine soil sample. The isolated strain was identified by 16s ribotyping method and it was found to be Streptomyces atrovirens . Furthermore, the synthesised AgNPs were characterised by various bio‐analytical techniques such as ultraviolet–visible spectrophotometer, atomic force microscopy, transmission electron microscopy, Fourier transform infra‐red spectroscopy, and X‐ray diffraction. Moreover, the results of 3‐(4, 5‐dimethylthiazol‐2‐yl)‐2, 5‐diphenyltetrazolium bromide assay reveals 44.51 µg of AgNPs to have profound inhibition of cancer cell growth; furthermore, the inhibition of MCF‐7 breast cancer cell line was found to be dose dependent on treatment with AgNPs. Acridine orange and ethidium bromide double staining methods were performed for cell morphological analysis. The present results showed that biosynthesised AgNPs might be emerging alternative biomaterials for human breast cancer therapy.