Actinobacterial‐mediated synthesis of silver nanoparticles and their activity against pathogenic bacteria. Issue 3 (8th September 2016)

• Record Type: Journal Article
• Authors: Wypij, Magdalena; Golinska, Patrycja; Dahm, Hanna; Rai, Mahendra
• Is Part Of: IET nanobiotechnology. Volume 11:Issue 3(2017)
• Page Start: 336
• Page End: 342
• Publication Date: 2016-09-08
• Subjects: antibacterial activity -- silver -- nanoparticles -- electrokinetic effects -- Fourier transform infrared spectra -- microorganisms -- nanofabrication; actinobacterial mediated synthesis -- silver nanoparticles -- pathogenic bacteria -- biosynthesis -- acidophilic actinobacterial SH11 strain -- pine forest soil -- 16S rRNA gene sequence -- Streptomyces kasugaensis M338‐M1T -- S. celluloflavus NRRL B‐2493T -- UV–visible spectroscopy -- transmission electron microscopy -- Fourier transform infrared spectroscopy -- zeta potential -- gram positive bacteria -- Staphylococcus aureus -- Bacillus subtilis -- gram negative bacteria -- Escherichia coli -- disc diffusion -- wavelength 420 nm -- Ag; Biotechnology -- Periodicals; Nanotechnology -- Periodicals; 660.6
• DOI: 10.1049/iet-nbt.2016.0112
• Languages: English
• ISSNs: 1751-8741

Abstract

Abstract : In this study, silver nanoparticles (AgNPs) were biosynthesised by using acidophilic actinobacterial SH11 strain isolated from pine forest soil. Isolate SH11 was identified based on 16S rRNA gene sequence to Streptomyces kasugaensis M338‐M1 T and S . celluloflavus NRRL B‐2493 T (99.8% similarity, both). Biosynthesised AgNPs were analysed by UV–visible spectroscopy, which revealed specific peak at λ = 420 nm. Transmission electron microscopy analyses showed polydispersed, spherical nanoparticles with a mean size of 13.2 nm, while Fourier transform infrared spectroscopy confirmed the presence of proteins as the capping agents over the surface of AgNPs. The zeta potential was found to be −16.6 mV, which indicated stability of AgNPs. The antibacterial activity of AgNPs from SH11 strain against gram‐positive ( Staphylococcus aureus and Bacillus subtilis ) and gram‐negative ( Escherichia coli ) bacteria was estimated using disc diffusion, minimum inhibitory concentration and live/dead analyses. The AgNPs showed the maximum antimicrobial activity against E. coli, followed by B. subtilis and S. aureus . Further, the synergistic effect of AgNPs in combination with commercial antibiotics (kanamycin, ampicillin, tetracycline) was also evaluated against bacterial isolates. The antimicrobial efficacy of antibiotics was found to be enhanced in the presence of AgNPs.