Specific interactions between functionalised particles and circulating tumour cells. Issue 1 (1st March 2014)

• Record Type: Journal Article
• Authors: Stamm, Matthew T.; Trickey‐Glassman, Andrew S.; Jiang, Linan; Zohar, Yitshak
• Is Part Of: IET nanobiotechnology. Volume 8:Issue 1(2014)
• Page Start: 18
• Page End: 23
• Publication Date: 2014-03-01
• Subjects: cellular biophysics -- silicon compounds -- tumours; functionalised particle interactions -- circulating tumour cells -- receptorâ€"ligand binding -- silica particles -- EpCAM antibodies -- EpCAM‐expressing BT‐20 breast cancer cells -- incubation time -- particle concentration -- functionalised particles -- bound particles -- binding affinity loss -- cellâ€"particleâ€"cell interaction -- cell‐surface coverage -- cellular binding affinity -- SiO2; Biotechnology -- Periodicals; Nanotechnology -- Periodicals; 660.6
• DOI: 10.1049/iet-nbt.2013.0034
• Languages: English
• ISSNs: 1751-8741

Abstract

Abstract : Receptor–ligand binding has been one of the more popular approaches to specifically targeting tumour cells. In this work, targeting efficiency was quantitatively characterized using silica particles functionalized with EpCAM antibodies and EpCAM‐expressing BT‐20 breast cancer cells. The effects of incubation time and particle concentration on the number of functionalised particles bound to target cells were experimentally investigated. The number of bound particles was found to increase with particle concentration, but not necessarily with incubation time. Binding affinity loss because of cell–particle–cell interaction was identified as a limiting mechanism for the number of particles bound to target cells. While cell‐surface coverage because of bound particles rises exponentially under low particle concentration, it features a peak value at high particle concentration. The current findings suggest that separation of a bound particle from a cell may be detrimental to cellular binding affinity.