Development of colorimetric cholesterol detection kit using TPU nanofibre/cellulose acetate membrane. Issue 5 (28th February 2018)

• Record Type: Journal Article
• Authors: Immanuel, Susan; Elakkiya, Venugopal; Alagappan, Muthuppalaniappan; Selvakumar, Rajendran
• Is Part Of: IET nanobiotechnology. Volume 12:Issue 5(2018)
• Page Start: 557
• Page End: 561
• Publication Date: 2018-02-28
• Subjects: colorimetry -- nanofibres -- biomembranes -- biosensors -- nanosensors -- nanofabrication -- polymer fibres -- elasticity -- nanomechanics -- biomechanics -- blood -- nanomedicine -- biomedical equipment -- enzymes -- molecular biophysics -- molecular configurations -- biochemistry -- abrasion -- biomedical materials -- electrospinning -- scanning electron microscopy -- Fourier transform infrared spectra -- dyes -- oxidation -- spectrophotometry; colorimetric cholesterol detection kit -- TPU nanofibre‐cellulose acetate membrane -- thermoplastic polyurethane nanofibres‐based kit -- highly elastic polymer -- microorganisms -- abrasion -- blood compatibility -- natural selection -- immobilisation matrix -- cholesterol oxidase ChOx enzyme -- electrospinning -- scanning electron microscopy -- Fourier transform‐infrared spectroscopy -- TPU nanofibre -- cholesterol level‐concentration -- 4‐aminoantipyrine -- H2 O2 production -- oxidation -- cellulose acetate membrane -- enzyme immobilisation matrix -- enzyme concentration -- optimisation -- dye quantity -- standard ChOx spectrophotometric assay -- enzyme immobilised nanofibre -- serum samples; Biotechnology -- Periodicals; Nanotechnology -- Periodicals; 660.6
• DOI: 10.1049/iet-nbt.2017.0246
• Languages: English
• ISSNs: 1751-8741

Abstract

Abstract : In this study, the authors report a simple fabrication of thermoplastic polyurethane (TPU) nanofibres‐based kit for cholesterol detection. TPU is a polymer that is highly elastic, resistant to microorganisms, abrasion and compatible with blood; thus, making it a natural selection as an immobilisation matrix for cholesterol oxidase (ChOx) enzyme. The nanofibre was fabricated by electrospinning process and was characterised using scanning electron microscopy and Fourier transform‐infrared spectroscopy. ChOx was covalently immobilised on TPU nanofibre and cholesterol level/concentration was visually found using 4‐aminoantipyrine, a dye that reacts with H2 O2 produced from the oxidation of cholesterol by ChOx and changes colour from yellow to red. The efficacy of the nanofibre to act as a detecting substrate was compared with cellulose acetate (CA) membrane, a well‐documented enzyme immobilisation matrix. The optimisation of enzyme concentration and dye quantity were performed using standard ChOx spectrophotometric assay and the same was used in CA membrane and TPU nanofibre. The ChOx immobilised nanofibre showed good linear range from 2 to 10 mM with a lower detection limit of 2 mM and was highly stable compared to that of CA membrane. The enzyme immobilised nanofibre was further validated in serum samples.