Synthesis, radiolabelling, and biological assessment of folic acid‐conjugated G‐3 99m Tc‐dendrimer as the breast cancer molecular imaging agent. Issue 7 (1st September 2020)

• Record Type: Journal Article
• Authors: Zamani, Saedeh; Shafeie‐Ardestani, Mehdi; Bitarafan‐Rajabi, Ahmad; Khalaj, Ali; Sabzevari, Omid
• Is Part Of: IET nanobiotechnology. Volume 14:Issue 7(2020)
• Page Start: 628
• Page End: 634
• Publication Date: 2020-09-01
• Subjects: toxicology -- single photon emission computed tomography -- technetium -- cancer -- bone -- polymers -- biochemistry -- tumours -- electrospinning -- biomedical materials -- light scattering -- cellular biophysics -- Fourier transform infrared spectra -- proton magnetic resonance -- transmission electron microscopy -- biological organs; biodistribution -- toxicity assessment -- cellular toxicity -- bright SPECT image -- PEG‐citrate dendrimer -- breast cancer molecular imaging agent -- proton nuclear magnetic resonance -- dendrimer‐based imaging agent -- folic acid‐conjugated G‐399m Tc‐dendrimer -- dendrimer‐G3 -- poly(ethylene glycol)‐citrate -- Fourier transform infrared spectra -- dynamic light scattering -- transition electron microscopy -- 2, 3‐bis‐(2‐methoxy‐4‐nitro‐5‐sulfophenyl)‐2H‐tetrazolium‐5‐carboxanilide cytotoxicity assay -- human serum -- tumour detection; Biotechnology -- Periodicals; Nanotechnology -- Periodicals; 660.6
• DOI: 10.1049/iet-nbt.2020.0176
• Languages: English
• ISSNs: 1751-8741

Abstract

Abstract : Hence, in this study, the authors aimed to develop a dendrimer‐based imaging agent comprised of poly(ethylene glycol) (PEG)‐citrate, technetium‐99 m ( 99m Tc), and folic acid. The dendrimer‐G3 was synthesised and conjugated with folic acid, which confirmed by Fourier transform infrared, proton nuclear magnetic resonance, dynamic light scattering, and transition electron microscopy. 2, 3‐bis‐(2‐methoxy‐4‐nitro‐5‐sulfophenyl)‐2H‐Tetrazolium‐5‐Carboxanilide cytotoxicity assay kit was used to measure the cellular toxicity of dendrimer. Imaging and biodistribution studies were conducted on the mice bearing tumour. The results showed that the fabricated dendrimer‐G3 has a size of 90 ± 3 nm, which was increased to 100 ± 4 nm following the conjugation with folic acid. The radiostablity investigation showed that the fabricated dendrimers were stable in the human serum at various times. Toxicity assessment confirmed no cellular toxicity against HEK‐293 cells at 0.25, 0.5, 1, 2, 4, and 8 mg/μl concentrations. The in vivo studies demonstrated that the synthesised dendrimers were able to provide a bright SPECT image applicable for tumour detection. In conclusion, the authors' study documented the positive aspects of PEG‐citrate dendrimer conjugated with folic acid as the SPECT contrast agent for breast cancer detection.