Synthesis of extracellular gold nanoparticles using Cupriavidus metallidurans CH34 cells. Issue 1 (31st October 2017)

• Record Type: Journal Article
• Authors: Montero‐Silva, Francisco; Durán, Nelson; Seeger, Michael
• Is Part Of: IET nanobiotechnology. Volume 12:Issue 1(2018)
• Page Start: 40
• Page End: 46
• Publication Date: 2017-10-31
• Subjects: gold -- nanoparticles -- cellular biophysics -- X‐ray diffraction -- transmission electron microscopy -- X‐ray chemical analysis -- Fourier transform infrared spectra -- proteins -- molecular biophysics -- light scattering -- colloids -- pH -- genomics -- nanomedicine -- antibacterial activity -- biomedical materials -- nanofabrication -- biochemistry; extracellular gold nanoparticles -- Cupriavidus metallidurans CH34 cells -- gold colloid -- bacterial biomass -- bacterial secretomes -- Au(III) ions -- extracellular dispersions -- powder X‐ray diffraction -- face‐centred cubic phase -- transmission electron microscopy -- AuNP morphology -- triangular nanostructures -- decahedral nanostructures -- energy dispersive X‐ray spectroscopy -- Fourier transform infrared spectra -- proteins -- dynamic light‐scattering measurements -- DLS measurements -- NP tracking analysis -- electron microscopy images -- biogenic colloid -- high ionic strength -- pH conditions -- bioinformatic analysis -- genomes -- Au; Biotechnology -- Periodicals; Nanotechnology -- Periodicals; 660.6
• DOI: 10.1049/iet-nbt.2017.0185
• Languages: English
• ISSNs: 1751-8741

Abstract

Abstract : In this study, spontaneous synthesis of a gold (Au) colloid using cells of Cupriavidus metallidurans CH34 is reported, and compared with results obtained using cells of the model bacterium Escherichia coli MG1655. To investigate the synthesis mechanism, bacterial biomass and secretomes from both strains were incubated with Au(III) ions. Only CH34 cells were capable of producing extracellular dispersions of Au nanoparticles (NPs). Transmission electron microscopy images showed that AuNPs morphology was dominated by triangular and decahedral nanostructures. Energy dispersive X‐ray spectroscopy and Fourier transform infrared spectra showed the presence of sulphur and vibrations associated to proteins. Average AuNPs diameter was obtained by dynamic light‐scattering measurements (DLS), NP tracking analysis measurements and analysis of electron microscopy images. Moreover, DLS measurements showed that the biogenic colloid was stable after exposure to ultrasound, high ionic strength and extreme pH conditions. The biogenic AuNPs produced by strain CH34 did not show antibacterial activity, in contrast to biogenic silver NPs. Comparative bioinformatic analysis of genomes from strain CH34 and strain MG1655 showed potential CH34 proteins that may be electron donors during reduction of Au(III) ions. On the basis of these results, a mechanism for the extracellular Au reduction by strain CH34 is proposed.