Biofabrication of broad range antibacterial and antibiofilm silver nanoparticles. Issue 5 (1st October 2016)

• Record Type: Journal Article
• Authors: Qayyum, Shariq; Khan, Asad Ullah
• Is Part Of: IET nanobiotechnology. Volume 10:Issue 5(2016)
• Page Start: 349
• Page End: 357
• Publication Date: 2016-10-01
• Subjects: silver -- nanoparticles -- antibacterial activity -- nanofabrication -- microorganisms -- ultraviolet spectra -- visible spectra -- transmission electron microscopy -- Fourier transform infrared spectra -- X‐ray diffraction -- proteins -- DNA -- nanomedicine -- biomedical materials -- cellular biophysics; biofabrication -- broad range antibacterial nanoparticles -- antibiofilm silver nanoparticles -- plant extract contribution -- drug resistant microbes -- UV‐vis spectroscopy -- transmission electron microscope -- Fourier transform infrared spectroscopy -- X‐ray diffraction -- Image J software -- resistant strains -- susceptible strains -- Gram positive bacteria -- fungus -- protein leakage assays -- carbohydrate leakage assays -- cell lysis -- DNA damage -- Ag; Biotechnology -- Periodicals; Nanotechnology -- Periodicals; 660.6
• DOI: 10.1049/iet-nbt.2015.0091
• Languages: English
• ISSNs: 1751-8741

Abstract

Abstract : Silver nanoparticles (AgNPs) were biosynthesized via a green route using ten different plants extracts (GNP1‐ Caryota urens, GNP2‐ Pongamia glabra, GNP3‐ Hamelia patens, GNP4‐ Thevetia peruviana, GNP5‐ Calendula officinalis, GNP6‐ Tectona grandis, GNP7‐ Ficus petiolaris, GNP8‐ Ficus busking, GNP9‐ Juniper communis, GNP10‐ Bauhinia purpurea ). AgNPs were tested against drug resistant microbes and their biofilms. These nanoparticles (NPs) were characterised using UV‐vis spectroscopy, transmission electron microscope, Fourier transform infrared spectroscopy, X‐ray diffraction and Image J software. Most of the AgNPs were distributed over a range of 1 of 60 nm size. The results indicated that AgNPs were antibacterial in nature without differentiating between resistant or susceptible strains. Moreover, the effect was more prominent on Gram negative bacteria then Gram positive bacteria and fungus. AgNPs inhibited various classes of microbes with different concentration. It was also evident from the results that the origin or nature of extract did not affect the activity of the NPs. Protein and carbohydrate leakage assays confirmed that the cells lysis is one of the main mechanisms for the killing of microbes by green AgNPs. This study suggests that the action of AgNPs on microbial cells resulted into cell lysis and DNA damage. Excellent microbial biofilm inhibition was also seen by these green AgNPs. AgNPs have proved their candidature as a potential antibacterial and antibiofilm agent against MDR microbes.