Facile nano‐free electrochemiluminescence biosensor for detection of sulphamethoxazole via tris(2, 2′‐bipyridyl)ruthenium(II) and N ‐methyl pyrrolidone recognition. Issue 2 (24th January 2020)

• Record Type: Journal Article
• Authors: Tong, Xiyuan; Jin, Shiyao; Zhao, Yingdai; Gai, Yue; E, Yifeng; Li, Dan
• Is Part Of: IET nanobiotechnology. Volume 14:Issue 2(2020)
• Page Start: 167
• Page End: 171
• Publication Date: 2020-01-24
• Subjects: biosensors -- electrochemical sensors -- electroluminescence -- chemiluminescence -- organic compounds -- electrochemistry; ruthenium complex -- analytical chemistry -- nonaqueous ECL luminescence system -- complex nanomodification -- quenching effect -- ECL signal quenching intensity -- ECL sensor system -- nanofree electrochemiluminescence biosensor system -- sulphamethoxazole detection -- tris(2, 2′‐bipyridyl)ruthenium(II) -- N‐methyl pyrrolidone recognition -- analyte determination -- nanomaterials -- SMZ concentration detection; Biotechnology -- Periodicals; Nanotechnology -- Periodicals; 660.6
• DOI: 10.1049/iet-nbt.2019.0257
• Languages: English
• ISSNs: 1751-8741

Abstract

Abstract : The electrochemiluminescence (ECL) system based on the ruthenium complex has become a powerful tool in the field of analytical chemistry. However, the non‐aqueous ECL luminescence system, which does not involve complex nano‐modification, has not been widely used for the determination of analytes. In this study, N ‐methyl pyrrolidone was selected as the solvent, and it could also act as a co‐reactant of Ru bpy 3 2 + . Based on this, a simple ECL system without nanomaterials was established. Strong ECL was generated. Furthermore, a quenching effect between the excited state of Ru bpy 3 2 + and sulphamethoxazole (SMZ) was observed. Based on this, a sensitive ECL sensor for detecting SMZ is constructed. A linear relationship between ECL signal quenching intensity (Δ I ) and the logarithm of SMZ concentration (log C ) in the concentration range of 1 × 10 −7 –1 × 10 −5 mol/l is obtained. The limit of detection is as low as 3.33 × 10 −9 mol/l. The method has been applied to the detection of SMZ in tap water samples with different concentration levels with satisfactory results, and the recovery was 95.3–102.6%.