High yield gold nanoparticle‐based DNA isolation method for human papillomaviruses genotypes from cervical cancer tissue samples. Issue 7 (5th August 2020)

• Record Type: Journal Article
• Authors: Seyyedi, Noorossadat; Farjadian, Fatemeh; Farhadi, Ali; Rafiei Dehbidi, Gholamreza; Ranjbaran, Reza; Zare, Farahnaz; Ali Okhovat, Mohammad; Nikouyan, Negin; Behzad‐Behbahani, Abbas
• Is Part Of: IET nanobiotechnology. Volume 14:Issue 7(2020)
• Page Start: 555
• Page End: 562
• Publication Date: 2020-08-05
• Subjects: cancer -- biological tissues -- microorganisms -- gold -- DNA -- lab‐on‐a‐chip -- gynaecology -- purification -- biochemistry -- molecular biophysics -- nanoparticles -- nanomedicine -- cellular biophysics; specific extraction method -- cervical cancer tissue samples -- high‐risk human papillomavirus infection -- paraffin‐embedded tissue samples -- specific DNA purification method -- sensitive DNA purification method -- FFPE tissue -- HR‐HPV DNA targets -- specific DNA sequences -- HR‐HPV genotypes -- type‐specific HPV DNA -- AuNP‐probe purification method -- gold nanoparticle‐based DNA isolation method -- oligonucleotide -- human papillomaviruse genotypes -- clinical samples -- Au; Biotechnology -- Periodicals; Nanotechnology -- Periodicals; 660.6
• DOI: 10.1049/iet-nbt.2020.0093
• Languages: English
• ISSNs: 1751-8741

Abstract

Abstract : Gold nanoparticles (AuNPs) are commonly used in biosensors of various kinds. However, its application to extract DNA from cancer tissues has not been extensively studied. The purification of DNA from cancer tissues is an important step in diagnostic and therapeutic development. Almost, all cervical cancer cases are associated with high‐risk human papillomavirus (HR‐HPV) infection. Accurate viral diagnosis has so far relied on the extraction of adequate amounts of DNA from formalin‐fixed, paraffin‐embedded (FFPE) tissue samples. Till now, no specific and sensitive DNA purification method has been introduced for the extraction of HR‐HPV from FFPE tissue. Since the commercially available purification kits are not sensitive and specific enough for HR‐HPV DNA targets, in this study, a DNA purification method was designed based on AuNPs to purify sufficient amounts of HR‐HPV DNA from cervical cancer tissue samples. AuNPs were coated with a series of oligonucleotide probes to hybridize to specific DNA sequences of HR‐HPV genotypes. Results showed that 733 out of 800 copies of type‐specific HPV DNA were recovered with complete specificity, compared to 36 copies with a standard commercial kit (Qiagen FFPE). The high yield of DNA (91.6%) is the main advantage of the AuNPs‐probe purification method.