Development of nanoformulation of picroliv isolated from Picrorrhiza kurroa. Issue 3 (1st June 2016)

• Record Type: Journal Article
• Authors: Guliani, Anika; Kumari, Avnesh; Kumar, Dharmesh; Yadav, Sudesh Kumar
• Is Part Of: IET nanobiotechnology. Volume 10:Issue 3(2016)
• Page Start: 114
• Page End: 119
• Publication Date: 2016-06-01
• Subjects: drugs -- nanomedicine -- nanofabrication -- biodegradable materials -- nanoparticles -- biomedical materials -- evaporation -- ultraviolet spectra -- visible spectra -- atomic force microscopy -- transmission electron microscopy -- Fourier transform infrared spectra -- electrokinetic effects -- drug delivery systems -- cellular biophysics -- toxicology; nanoformulation development -- Picrorrhiza kurroa -- picroside I‐kutkoside mixture -- rhizome -- pharmaceutical properties -- hepatoprotective activity -- anticholestatic activity -- antioxidant activity -- immune‐modulating activity -- aqueous solubility -- bioavailability -- biodegradable poly lactic acid nanoparticles -- solvent evaporation method -- picroliv‐loaded PLA NPs -- UV‐visible spectroscopy -- atomic force microscopy -- transmission electron microscopy -- Fourier transform infrared spectra -- zeta‐sizer -- in vitro picroliv release -- KB cell lines -- initial burst release -- cytotoxic activity -- picroliv nanoformulation -- pharma‐sector; Biotechnology -- Periodicals; Nanotechnology -- Periodicals; 660.6
• DOI: 10.1049/iet-nbt.2015.0032
• Languages: English
• ISSNs: 1751-8741

Abstract

Abstract : Picroliv, a mixture of picroside I and kutkoside isolated from rhizome of Picrorrhiza kurroa has been reported for many pharmaceutical properties such as hepatoprotective, anticholestatic, antioxidant and immune‐modulating activity. However, picroliv possessed lesser efficacy due to its poor aqueous solubility and lesser bioavailability. To find solution, picroliv was loaded into biodegradable poly lactic acid nanoparticles (PLA NPs) using solvent evaporation method. The picroliv‐loaded PLA NPs were characterised by UV–vis spectroscopy, atomic force microscopy, transmission electron microscopy, Fourier transform infrared and Zeta sizer. The size of picroliv‐loaded PLA NPs was 182 ± 20 nm. Zeta potential of picroliv‐loaded PLA NPs was −23.5 mV, indicated their good stability. In vitro picroliv release from picroliv‐loaded PLA NPs showed an initial burst release followed by slow and sustained release. The efficacy of picroliv‐loaded PLA NPs was assessed against KB cell lines. Blank PLA NPs showed no cytotoxicity on KB cells. The picroliv‐loaded PLA NPs showed more cytotoxic activity on KB cells as compared to the pure drug. Hence, the developed picroliv nanoformulation would find potential application in pharma‐sector.