Theoretical and experimental studies on ionic currents in nanopore‐based biosensors. Issue 4 (1st December 2014)

• Record Type: Journal Article
• Authors: Liu, Lei; Li, Chu; Ma, Jian; Wu, Yingdong; Ni, Zhonghua; Chen, Yunfei
• Is Part Of: IET nanobiotechnology. Volume 8:Issue 4(2014)
• Page Start: 247
• Page End: 256
• Publication Date: 2014-12-01
• Subjects: biological fluid dynamics -- biosensors -- Boltzmann equation -- DNA -- ionic conductivity -- molecular biophysics -- nanobiotechnology -- nanoporous materials -- Navier‐Stokes equations -- Poisson equation -- nanofluidics; nanopore‐based biosensors -- nanofluidic devices -- low‐cost DNA sequencing -- translocation -- ion concentration -- ionic flowing speed -- ionic current -- Poisson‐Boltzmann equation -- Navier‐Stokes equation -- Nernst‐Planck equation; Biotechnology -- Periodicals; Nanotechnology -- Periodicals; 660.6
• DOI: 10.1049/iet-nbt.2013.0017
• Languages: English
• ISSNs: 1751-8741

Abstract

Abstract : Novel generation of analytical technology based on nanopores has provided possibilities to fabricate nanofluidic devices for low‐cost DNA sequencing or rapid biosensing. In this paper, a simplified model was suggested to describe DNA molecule's translocation through a nanopore, and the internal potential, ion concentration, ionic flowing speed and ionic current in nanopores with different sizes were theoretically calculated and discussed on the basis of Poisson–Boltzmann equation, Navier–Stokes equation and Nernst–Planck equation by considering several important parameters, such as the applied voltage, the thickness and the electric potential distributions in nanopores. In this way, the basic ionic currents, the modulated ionic currents and the current drops induced by translocation were obtained, and the size effects of the nanopores were carefully compared and discussed based on the calculated results and experimental data, which indicated that nanopores with a size of 10 nm or so are more advantageous to achieve high quality ionic current signals in DNA sensing.