Biosynthesis of SeNPs by Mycobacterium bovis and their enhancing effect on the immune response against HBs antigens: an in vivo study. Issue 1 (1st November 2017)

• Record Type: Journal Article
• Authors: Mavandadnejad, Faranak; Yazdi, Mohammad Hossein; Hassanzadeh, Seyed Mehdi; Mahdavi, Mehdi; Faramarzi, Mohammad Ali; Pazoki‐Toroudi, Hamidreza; Shahverdi, Ahmad Reza
• Is Part Of: IET nanobiotechnology. Volume 12:Issue 1(2018)
• Page Start: 57
• Page End: 63
• Publication Date: 2017-11-01
• Subjects: selenium -- nanoparticles -- nanofabrication -- microorganisms -- Fourier transform spectra -- infrared spectra -- light scattering -- atomic force microscopy -- X‐ray chemical analysis -- transmission electron microscopy -- thermal analysis -- nanomedicine; biosynthesis -- selenium nanoparticles -- Mycobacterium bovis -- enhnced immune response -- HBs antigens -- Bacillus Calmette‐Guerin -- immune modulators -- biogenic SeNPs -- immune response pattern -- Sauton medium broth -- Fourier transform infrared spectroscopy -- dynamic light scattering -- atomic force microscopy -- energy dispersive X‐ray spectrum -- transmission electron microscopy -- thermogravimetric analysis -- female inbred BALB/c mice -- antibody -- IgG1 isotype -- IgG2a isotype -- IL4 isotype -- interferon‐γ -- enzyme‐linked immunosorbent assay -- bacterium -- time 24 h -- time 14 day -- time 28 day -- Se; Biotechnology -- Periodicals; Nanotechnology -- Periodicals; 660.6
• DOI: 10.1049/iet-nbt.2017.0006
• Languages: English
• ISSNs: 1751-8741

Abstract

Abstract : Conventional hepatitis B vaccine contains alum but is less effective to induce Th1 response. Selenium nanoparticles and Bacillus Calmette–Guerin were reported as immune modulators. In this study, SeNPs were extracted from Mycobacterium bovis and characterised. SeNPs were mixed with HBs‐Ag and administered to the mice to investigate he immune response pattern. With an addition of Se ions at a sub‐inhibitory concentration to the Sauton medium broth after 24 h, SeNPs were extracted from M. bovis and characterised by Fourier transform infrared spectroscopy, dynamic light scattering, atomic forcemicroscopy, energy dispersive X‐ray spectrum, transmission electron microscopy, and thermogravimetric analysis. Furthermore, female inbred BALB/c mice were injected subcutaneously on the first, 14th, 28th day with 100 and 200 µg doses of that SeNPs supplemented with HBs‐Ag vaccine. Later, the total antibody, isotypes of Immunoglobulin G1, Interlukin 4, and interferon‐ γ were measured by enzye‐linked immunosorbent assay. The size of the SeNPs was <150 nm. Level of total antibody and immunoglobulin G2a increased significantly in the group that received 200 µg/ml nano selenium extracted from M. bovis . SeNPs in dose of 200 µg coated with organic materials of M. bovis could induce an influential immune response in relation to the conventional HBs‐Ag vaccine.