Culture-independent study of bacterial communities in tropical river sediment. Issue 1 (2nd January 2017)

• Record Type: Journal Article
• Authors: Thoetkiattikul, Honglada; Mhuantong, Wuttichai; Pinyakong, Onruthai; Wisawapipat, Worachart; Yamazoe, Atsushi; Fujita, Nobuyuki; Eurwilaichitr, Lily; Champreda, Verawat
• Is Part Of: Bioscience, biotechnology, and biochemistry. Volume 81:Issue 1(2017)
• Page Start: 200
• Page End: 209
• Publication Date: 2017-01-02
• Subjects: 16S rRNA gene -- aquatic environment -- bacterial diversity -- next generation sequencing -- river sediment; Biotechnology -- Periodicals; Biochemistry -- Periodicals; 660.6
• DOI: 10.1080/09168451.2016.1234927
• Languages: English
• ISSNs: 0916-8451

Abstract

Abstract: Ubiquitous microbial communities in river sediments actively govern organic matter decomposition, nutrient recycling, and remediation of toxic compounds. In this study, prokaryotic diversity in two major rivers in central Thailand, the Chao Phraya (CP) and the Tha Chin (TC) distributary was investigated. Significant differences in sediment physicochemical properties, particularly silt content, were noted between the two rivers. Tagged 16S rRNA sequencing on a 454 platform showed that the sediment microbiomes were dominated by Gammaproteobacteria and sulfur/sulfate reducing Deltaproteobacteria, represented by orders Desulfobacteriales and Desulfluromonadales together with organic degraders Betaproteobacteria (orders Burkholderiales and Rhodocyclales ) together with the co-existence of Bacteroidetes predominated by Sphingobacteriales . Enrichment of specific bacterial orders was found in the clayey CP and silt-rich TC sediments, including various genera with known metabolic capability on decomposition of organic matter and xenobiotic compounds. The data represent one of the pioneered works revealing heterogeneity of bacteria in river sediments in the tropics. Graphical abstract: : Analysis of bacterial diversity by tagged 16S rRNA sequencing revealed enrichment of specific taxa in CP and TC rivers.