Advancing full length genome sequencing for human RNA viral pathogens. (January 2009)

• Record Type: Journal Article
• Authors: Djikeng, Appolinaire; Spiro, David
• Is Part Of: Future virology. Volume 4:Number 1(2009)
• Page Start: 47
• Page End: 53
• Publication Date: 2009-01
• Subjects: emerging and re-emerging viruses -- genomics -- human viruses -- viral genome sequencing -- viral infectious diseases; Virology -- Periodicals; Virus diseases -- Treatment -- Periodicals; 616.9101
• DOI: 10.2217/17460794.4.1.47
• Languages: English
• ISSNs: 1746-0794

Abstract

In the face of numerous emerging and re-emerging viral threats, large-scale genome sequencing efforts are underway to monitor viral evolution in real-time. To fully appreciate the mechanisms of viral adaptation and evolution, and to also develop reagents and resources for a better molecular diagnosis of emerging and re-emerging viral infections, there has been an increasing effort toward producing full length viral genome sequences. To date, high-throughput platforms have been developed using traditional Sanger-based sequencing and there are currently prospects to apply next generation sequencing methods to develop an ultra high-throughput strategy for viral genome sequencing and analysis.