Background-free Detection of Mouse Antibodies on Mouse Tissue by Anti-isotype Secondary Antibodies. (August 2021)

• Record Type: Journal Article
• Authors: Mascadri, Francesco; Ciccimarra, Roberta; Bolognesi, Maddalena M.; Stellari, Fabio; Ravanetti, Francesca; Cattoretti, Giorgio
• Is Part Of: Journal of histochemistry and cytochemistry. Volume 69:Number 8(2021)
• Page Start: 535
• Page End: 541
• Publication Date: 2021-08
• Subjects: antibodies -- immunoglobulin isotypes -- immunohistochemistry -- indicators and reagents -- mice; Histochemistry -- Periodicals; Cytochemistry -- Periodicals; Histocytochemistry -- methods; Histological Techniques; 572.05
• DOI: 10.1369/00221554211033239
• Languages: English
• ISSNs: 0022-1554

Abstract

Immunodetection on mouse routinely processed tissue via antibodies raised in mice faces cross-reactivity of the secondary anti-mouse reagents with endogenous immunoglobulins, which permeate the tissue. Various solutions to this problem have been devised and include endogenous Ig block with anti-mouse Fab fragments or directly conjugated primary antibodies. Mouse isotype-specific antibodies, differently from reagents directed against both heavy and light chains, fail to detect endogenous Ig after fixation and embedding, while providing a clean and specific detection system for mouse antibodies on mouse routinely processed tissue.