Archaeal ribonuclease P proteins have potential for biotechnological applications where precise hybridization of nucleic acids is needed. Issue 12 (2nd December 2015)

• Record Type: Journal Article
• Authors: Miyanoshita, Mitsuru; Nakashima, Takashi; Kakuta, Yoshimitsu; Kimura, Makoto
• Is Part Of: Bioscience, biotechnology, and biochemistry. Volume 79:Issue 12(2015)
• Page Start: 2014
• Page End: 2017
• Publication Date: 2015-12-02
• Subjects: fluorescence resonance energy transfer -- RNA annealing -- RNase P proteins -- strand displacement; Biotechnology -- Periodicals; Biochemistry -- Periodicals; 660.6
• DOI: 10.1080/09168451.2015.1058699
• Languages: English
• ISSNs: 0916-8451

Abstract

Abstract: Fluorescence resonance energy transfer-based assay showed that archaeal ribonuclease P (RNase P) proteins significantly promoted DNA annealing and strand displacement. Moreover, we found that archaeal RNase P proteins could discriminate nucleotide exchanges in DNA chains via their activity accelerating DNA strand displacement, suggesting that they have potential for biotechnological application to genetic diagnosis.