Review of Stimulated Raman Scattering Microscopy Techniques and Applications in the Biosciences. Issue 1 (30th December 2020)

• Record Type: Journal Article
• Authors: Li, Yanping; Shen, Binglin; Li, Shaowei; Zhao, Yihua; Qu, Junle; Liu, Liwei
• Is Part Of: Advanced biology. Volume 5:Issue 1(2021)
• Page Start: n/a
• Page End: n/a
• Publication Date: 2020-12-30
• Subjects: biological imaging -- chemical bond vibration -- medical diagnosis -- Raman scattering microscopy -- Raman tags; Molecular biology -- Periodicals; Systems biology -- Periodicals; Biological systems -- Periodicals; Biotechnology -- Periodicals; Bioengineering -- Periodicals; Biomedical engineering -- Periodicals; 660.6
• DOI: 10.1002/adbi.202000184
• Languages: English
• ISSNs: 2701-0198

Abstract

Abstract: Stimulated Raman scattering (SRS) microscopy is a nonlinear optical imaging method for visualizing chemical content based on molecular vibrational bonds. Featuring high speed, high resolution, high sensitivity, high accuracy, and 3D sectioning, SRS microscopy has made tremendous progress toward biochemical information acquisition, cellular function investigation, and label‐free medical diagnosis in the biosciences. In this review, the principle of SRS, system design, and data analysis are introduced, and the current innovations of the SRS system are reviewed. In particular, combined with various bio‐orthogonal Raman tags, the applications of SRS microscopy in cell metabolism, tumor diagnosis, neuroscience, drug tracking, and microbial detection are briefly examined. The future prospects for SRS microscopy are also shared. Abstract : In this Review, the principle of stimulated Raman scattering (SRS), the current innovations of system design, and the data analysis are introduced. Combined with various bio‐orthogonal Raman tags, applications of SRS microscopy in cell metabolism, tumor diagnosis, neuroscience, drug tracking, microbial detection are briefly reviewed. New perspectives for SRS microscopy are presented.