Expression of DMP-1 in the human pulp tissue using low level laser therapy. (24th July 2015)

• Record Type: Journal Article
• Authors: Neto, Natalino Lourenço; Teixeira Marques, Nádia Carolina; Fernandes, Ana Paula; Rodini, Camila Oliveira; Silva, Thiago Cruvinel; Andrade Moreira Machado, Maria Aparecida; Oliveira, Thais Marchini
• Is Part Of: Laser physics. Volume 25:Number 9(2015:Sep.)
• Publication Date: 2015-07-24
• Subjects: laser therapy low level -- immunohistochemistry -- tooth deciduous -- dental pulp; Lasers -- Periodicals; 621.36605
• DOI: 10.1088/1054-660X/25/9/095601
• Languages: English
• ISSNs: 1054-660X

Abstract

Abstract: This study aimed to evaluate the effects of low-level laser therapy (LLLT) on DMP-1 expression in pulp tissue repair of human primary teeth. Twenty mandibular primary molars were randomly assigned into the following groups: Group I—Buckley's Formocresol (FC); Group II—Calcium Hydroxide (CH); Group III—LLLT + CH and Group IV—LLLT + Zinc oxide/Eugenol. The teeth at the regular exfoliation period were extracted for histological analysis and immunolocalization of DMP-1. Descriptive analysis was performed on the dentin pulp complex. Histopathological assessment showed internal resorption in group FC. Groups CH and LLLT + CH provided better pulpal repair due to the absence of inflammation and the formation of hard tissue barrier. These two groups presented odontoblastic layer expressing DMP-1. According to this study, low level laser therapy preceding the use of calcium hydroxide exhibited satisfactory bio-inductive activity on pulp tissue repair of human primary teeth. However, other histological and cellular studies are needed to confirm the laser tissue action and efficacy.