Engineered collagen-binding bone morphogenetic protein-2 incorporated with platelet-rich plasma accelerates lumbar fusion in aged rats with osteopenia. Issue 14 (July 2021)

• Record Type: Journal Article
• Authors: Zhu, Weiguo; Kong, Chao; Pan, Fumin; Ouyang, Miao; Sun, Kang; Lu, Shibao
• Is Part Of: Experimental biology and medicine. Volume 246:Issue 14(2021)
• Page Start: 1577
• Page End: 1585
• Publication Date: 2021-07
• Subjects: Osteopenia -- bone morphogenetic protein-2 -- collagen-binding -- sustained release -- platelet-rich plasma -- spinal fusion; Physiology -- Periodicals; Biology, Experimental -- Periodicals; Medicine, Experimental -- Periodicals; 610.72
• DOI: 10.1177/15353702211001039
• Languages: English
• ISSNs: 1535-3702

Abstract

In aged individuals, osteopenia is a great concern for achieving solid spinal fusion. Spinal malunion could lead to various implant-related complications and reduce postoperative quality of life. This study aims to investigate the efficacy of collagen-binding bone morphogenetic protein-2 (CBD-BMP-2) on the treatment of lumbar inter-transverse defects and to explore whether platelet-rich plasma could help CBD-BMP-2 to achieve a better outcome in terms of osteogenesis in senile rats with osteopenia. In vitro experiment proved the angiogenic function of platelet-rich plasma and osteogenic effect of CBD-BMP-2. Rats were performed posterolateral lumbar inter-transverse fusion. Rats implanted with CBD-BMP-2 + platelet-rich plasma were assigned to Group A ( n = 20), rats implanted with CBD-BMP-2 were assigned to Group B ( n = 20), and those with platelet-rich plasma were assigned to Group C ( n = 20). Four weeks after implantation, radiographic assessment, manual palpation, and histological evaluation were performed. In vivo experiments showed satisfactory therapeutic effect on lumbar inter-transverse fusion in both Groups A and B and better results of bone microarchitecture in Group A. Solid fusion rate was 77.8% in Group A, 66.7% in Group B, and 0% in Group C ( P < 0.001). Our study indicated that CBD-BMP-2 could effectively facilitate the lumbar inter-transverse fusion in aged rats with osteopenia and platelet-rich plasma could help CBD-BMP-2 to enhance the bone healing of vertebral defects.