A Photolabile Semiconducting Polymer Nanotransducer for Near‐Infrared Regulation of CRISPR/Cas9 Gene Editing. Issue 50 (30th October 2019)

• Record Type: Journal Article
• Authors: Lyu, Yan; He, Shasha; Li, Jingchao; Jiang, Yuyan; Sun, He; Miao, Yansong; Pu, Kanyi
• Is Part Of: Angewandte Chemie international edition. Volume 58:Issue 50(2019)
• Page Start: 18197
• Page End: 18201
• Publication Date: 2019-10-30
• Subjects: CRISPR/Cas9 -- gene editing -- nanotechnology -- photoregulation -- semiconducting polymers; Chemistry -- Periodicals; 540
• DOI: 10.1002/anie.201909264
• Languages: English
• ISSNs: 1433-7851

Abstract

Abstract: Noninvasive regulation of CRISPR/Cas9 gene editing is conducive to understanding of gene function and development of gene therapy; however, it remains challenging. Herein, a photolabile semiconducting polymer nanotransducer (pSPN) is synthesized to act as the gene vector to deliver CRISPR/Cas9 plasmids into cells and also as the photoregulator to remotely activate gene editing. pSPN comprises a 1 O2 ‐generating backbone grafted with polyethylenimine brushes through 1 O2 ‐cleavable linkers. NIR photoirradiation spontaneously triggers the cleavage of gene vectors from pSPN, resulting in the release of CRISPR/Cas9 plasmids and subsequently initiating gene editing. This system affords 15‐ and 1.8‐fold enhancement in repaired gene expression relative to the nonirradiated controls in living cells and mice, respectively. As this approach does not require any specific modifications on biomolecular components, pSPN represents the first generic nanotransducer for in vivo regulation of CRISPR/Cas9 gene editing. Abstract : Gene genie : A photolabile semiconducting polymer nanotransducer (pSPN) is synthesized not only to act as the gene vector to deliver plasmids into cells but also to behave as the photoregulator to remotely and noninvasively activate CRISPR/Cas9 gene editing.