14-3-3β/α-A interacts with glycoprotein of spring viremia of carp virus and positively affects viral entry. Issue 81 (October 2018) - Chen, Buxin
Abstract: Spring viremia of carp virus (SVCV) is a fierce pathogen causing high mortality in the common carp. The glycoprotein (G protein) of SVCV is a pivotal component of the viral structure, located in the surface of the virion, and plays a key role in viral endocytosis. In this study, tandem affinity purification (TAP) followed by mass spectrometry analysis (LC-MS/MS) was carried out to search for novel host molecules that interact with SVCV G protein and a 14-3-3β/α-A protein was identified. The level of 14-3-3β/α-A mRNA expression was dramatically down regulated by SVCV infection. Furthermore, over expression of 14-3-3β/α-A results in a significantly increased SVCV attachment and entry in FHM cells. This study reveals an important role of 14-3-3 protein in regulating the early stage of SVCV infection, which offers a potential target for development of anti-SVCV therapies. Highlights: A 14-3-3 protein interacted with SVCV-G protein which was identified by TBP-MS in the FHM cell. The mRNA and protein expression level of 14-3-3β/α-A were downregulated due to SVCV infection in FHM cell. Overexpression of 14-3-3β/α-A significantly enhanced SVCV entry in FHM cell.