Tissue inhibitor of metalloproteinase-1 and interleukin-10 in serum from naïve and scrapie infected sheep. (June 2019)

• Record Type: Journal Article
• Authors: Silva, Marta G.; Madsen-Bouterse, Sally A.; Dassanayake, Rohana P.; Mousel, Michelle; Knowles, Donald P.
• Is Part Of: Veterinary and animal science. Volume 7(2019)
• Publication Date: 2019-06
• Subjects: Sheep -- Scrapie -- Prion -- Tissue inhibitor of metalloproteinase-1 -- Interleukin-10 -- Biomarker
• DOI: 10.1016/j.vas.2019.100056
• Languages: English
• ISSNs: 2451-943X

Abstract

Highlights: The development of novel diagnostic methods to identify pre-clinical scrapie-infected sheep is desired. IL-10 and TIMP-1 were assessed in serum from naïve and scrapie-infected sheep by ELISA. TIMP-1 levels were significant lower in young naïve sheep compared with old naïve and old infected sheep ( P <0.04). Both cytokines tended to have lower levels in young naïve sheep compared to infected sheep. Abstract: Tissue inhibitor of metalloproteinase-1 (TIMP-1) and interleukin-10 (IL-10) were identified as potential biomarkers for ovine scrapie in a mouse model. The development of novel diagnostic methods to identify pre-clinical scrapie-infected animals is needed. In this study, ELISA was used to assess TIMP-1 and IL-10 levels in 158 serum samples from naïve and preclinical scrapie-infected sheep. Young (≤ 18 months) naïve sheep had significantly lower TIMP-1 levels compared with old (≥ 20 months) naïve and old infected sheep ( P <0.04). Young naïve sheep had lower IL-10 than old naïve sheep ( P <0.001). Both cytokines tended to have lower levels in young naïve sheep compared to infected sheep but this did not reach significance. A larger sample size will be helpful in determining the potential of these cytokines as a diagnostic tool.