Isolation and Functional Determination of SKOR Potassium Channel in Purple Osier Willow, Salix purpurea. (25th February 2021)

• Record Type: Journal Article
• Authors: Chen, Yahui; Peng, Xuefeng; Cui, Jijie; Zhang, Hongxia; Jiang, Jiang; Song, Zhizhong
• Other Names: Filiz Ertugrul Academic Editor.
• Is Part Of: International journal of genomics. Volume 2021(2021)
• Publication Date: 2021-02-25
• Subjects: Genomes -- Periodicals; Genomics -- Periodicals; Cytogenetics -- Periodicals; Genomics; Genome; Molecular Biology; Cytogenetics; Genomes; Genomics; Periodicals; 572.86
• DOI: 10.1155/2021/6669509
• Languages: English
• ISSNs: 2314-436X

Abstract

Abstract : Potassium (K + ) plays key roles in plant growth and development. However, molecular mechanism studies of K + nutrition in forest plants are largely rare. In plants, SKOR gene encodes for the outward rectifying Shaker-type K + channel that is responsible for the long-distance transportation of K + through xylem in roots. In this study, we determined a Shaker-type K + channel gene in purple osier ( Salix purpurea ), designated as SpuSKOR, and determined its function using a patch clamp electrophysiological system. SpuSKOR was closely clustered with poplar PtrSKOR in the phylogenetic tree. Quantitative real-time PCR (qRT-PCR) analyses demonstrated that SpuSKOR was predominantly expressed in roots, and expression decreased under K + depletion conditions. Patch clamp analysis via HEK293-T cells demonstrated that the activity of the SpuSKOR channel was activated when the cell membrane voltage reached at -10 mV, and the channel activity was enhanced along with the increase of membrane voltage. Outward currents were recorded and induced in response to the decrease of external K + concentration. Our results indicate that SpuSKOR is a typical voltage dependent outwardly rectifying K + channel in purple osier. This study provides theoretical basis for revealing the mechanism of K + transport and distribution in woody plants.