Preparation of gentiobiose-derived oligosaccharides by glucansucrase E81 and determination of prebiotic and immune-modulatory functions. (1st December 2019)

• Record Type: Journal Article
• Authors: İspirli, Hümeyra; Colquhoun, Ian J.; Şahin, Engin; Sagdic, Osman; Dertli, Enes
• Is Part Of: Carbohydrate research. Volume 486(2019)
• Publication Date: 2019-12-01
• Subjects: Glucansucrase -- Gentiobiose-derived oligosaccharides -- Immune modulation -- Prebiotic; Carbohydrates -- Periodicals; Chemistry, Organic -- Periodicals; Biochemistry -- Periodicals; Carbohydrates -- Periodicals; Chimie organique -- Périodiques; Glucides -- Périodiques; Biochemistry; Carbohydrates; Chemistry, Organic; Periodicals; Electronic journals; 507.78
• DOI: 10.1016/j.carres.2019.107837
• Languages: English
• ISSNs: 0008-6215

Abstract

Abstract: Gentiobiose-derived oligosaccharides were synthesized by the acceptor reaction of glucansucrase E81 obtained from Lactobacillus reuteri E81 with sucrose and gentiobiose as donor-acceptor sugars, respectively. The reaction products were monitored by TLC analysis and gentiobiose-derived oligosaccharides up to DP 8 were formed during the acceptor reaction as determined by ESI-MS/MS analysis. The glycosylation of the gentiobiose with α-(1 → 6) linkages and α-(1 → 3) linkages was shown by 1 H and 13 C NMR analysis confirming the structure of these gentiobiose-derived oligosaccharides. The in vitro prebiotic function of the oligosaccharides was determined in which probiotic strains were stimulated whereas no growth was observed in pathogen strains. Gentiobiose-derived oligosaccharides showed immune-modulatory functions in vitro and triggered the production of IL-4, IL12 and TNF-α cytokines in HT29 cells in a dose dependent manner. This study showed the production and functional characterisation of gentiobiose-derived oligosaccharides establishing a promising avenue for future applications. Graphical abstract: Image 1 Highlights: Gentio-oligosaccharides produced by glucansucrase E81. Glucansucrase E81 transferred (1, 6)Glc and (1, 3)Glc units to gentiobiose. Up to DP 8 oligosaccharides produced from gentiobiose. Gentio-oligosaccharides showed prebiotic functions. All cytokines tested except IL-10 induced with gentio-oligosaccharides in vitro .