Pre-β1 HDL in type 2 diabetes mellitus. (August 2017)

• Record Type: Journal Article
• Authors: Shiu, S.W.; Wong, Y.; Tan, K.C.
• Is Part Of: Atherosclerosis. Volume 263(2017)
• Page Start: 24
• Page End: 28
• Publication Date: 2017-08
• Subjects: Pre-β1 HDL -- ATP-binding cassette transporter A1 -- Cholesterol efflux -- Type 2 diabetes -- Cardiovascular disease; Arteriosclerosis -- Periodicals; Electronic journals; 616.136
• DOI: 10.1016/j.atherosclerosis.2017.05.031
• Languages: English
• ISSNs: 0021-9150

Abstract

Abstract: Background and aims: Pre-β1 HDL, being a major acceptor of free cholesterol from cells, plays an important role in reverse cholesterol transport. This study was performed to determine whether abnormalities in pre-β1 HDL concentration were present in type 2 diabetes irrespective of their HDL-cholesterol levels, and the impact on cholesterol efflux. Methods: 640 type 2 diabetic patients with or without cardiovascular disease (CVD) and 360 non-diabetic controls matched for serum HDL-cholesterol levels were recruited. Plasma pre-β1 HDL was measured by ELISA, and cholesterol efflux to serum, mediated by ATP-binding cassette transporter A1 (ABCA1), was determined by measuring the transfer of [3H]cholesterol from cultured cells expressing ABCA1 to the medium containing the tested serum. Results: Despite the diabetic subjects having matched HDL-cholesterol and total apoA1 as controls, plasma pre-β1 HDL was significantly reduced in both male ( p < 0.01) and female diabetic patients ( p < 0.05), and patients with CVD had the lowest pre-β1 HDL level. Serum capacity to induce ABCA1-mediated cholesterol efflux was impaired in the diabetic group ( p < 0.01) and cholesterol efflux correlated with pre-β1 HDL (Pearson's r = 0.38, p < 0.01), and this association remained significantly even after controlling for age, gender, body mass index, diabetes status, smoking, apoA1, triglyceride and LDL. Conclusions: Plasma pre-β1 HDL level was significantly decreased in type 2 diabetes and was associated with a reduction in cholesterol efflux mediated by ABCA1. Our data would suggest that low pre-β1 HDL might cause impairment in reverse cholesterol transport in type 2 diabetes. Highlights: Pre-β1 HDL was reduced in type 2 diabetes despite having similar HDL-C as control. ABCA1-mediated cholesterol efflux to serum was impaired in type 2 diabetes. Low pre-β1 HDL level was associated with impaired cholesterol efflux.