Enhanced methane production from wheat straw with the assistance of lignocellulolytic microbial consortium TC-5. (September 2018)

• Record Type: Journal Article
• Authors: Kong, Xiangping; Du, Jing; Ye, Xiaomei; Xi, Yonglan; Jin, Hongmei; Zhang, Min; Guo, Dong
• Is Part Of: Bioresource technology. Volume 263(2018)
• Page Start: 33
• Page End: 39
• Publication Date: 2018-09
• Subjects: Lignocellulose degradation -- Microbial consortium -- Wheat straw -- Methane -- Anaerobic digestion; Biomass -- Periodicals; Biomass energy -- Periodicals; Bioremediation -- Periodicals; Agricultural wastes -- Periodicals; Factory and trade waste -- Periodicals; Organic wastes -- Periodicals; Bioénergie -- Périodiques; Déchets agricoles -- Périodiques; Déchets industriels -- Périodiques; Déchets organiques -- Périodiques; Déchets (Combustible) -- Périodiques; 662.88
• DOI: 10.1016/j.biortech.2018.04.079
• Languages: English
• ISSNs: 0960-8524

Abstract

Highlights: Consortium TC-5 showed a variety of lignocellulolytic enzymes activity. Un-pretreated wheat straw was degraded by TC-5 with a high ratio of 45.7%. Methane production was enhanced under mesophilic and thermophilic conditions. Abstract: The major obstacle of methane production from lignocellulose lies in the inefficient deconstruction of biomass. In this study, an anaerobic microbial consortium TC-5 was enriched with high lignocellulose-degradation capacity to enhance methane production from wheat straw. High degradation ratio of 45.7% of un-pretreated wheat straw was achieved due to a multi-species lignocellulolytic enzyme presented in the crude culture supernatant. The specific activity of xylanase, xylan esterase and β-xylosidase reached the highest level of 4.23, 0.15 and 0.48 U/mg, while cellobiohydrolase, endoglucanase and β-glucosidase showed the highest specific activity of 0.36, 0.22 and 0.41 U/mg during 9 days' degradation. Inoculation of TC-5 in digestion sludge during anaerobic digestion of wheat straw resulted in remarkable enhancement of 22.2% and 36.6% in methane yield under mesophilic and thermophilic conditions, respectively. This work demonstrates the potential of TC-5 for enhancing the production of biogas and other chemicals through biomass based biorefinery.