Pilot study to investigate the possibility of cytogenetic and physiological changes in bio-electrosprayed human lymphocyte cells. (May 2008)

• Record Type: Journal Article
• Authors: Kempski, Helena; Austin, Nicola; Roe, Amy; Chatters, Steve; Jayasinghe, Suwan N
• Is Part Of: Regenerative medicine. Volume 3:Number 3(2008)
• Page Start: 343
• Page End: 349
• Publication Date: 2008-05
• Subjects: aerodynamically assisted bio-jetting/threading -- bio-electrosprays -- cell electrospinning -- human blood cells -- karyotyping; Cellular therapy -- Periodicals; Stem cells -- Transplantation -- Periodicals; Degeneration (Pathology) -- Treatment -- Periodicals; Regeneration (Biology) -- Periodicals; 611.018
• DOI: 10.2217/17460751.3.3.343
• Languages: English
• ISSNs: 1746-0751

Abstract

Background: We recently pioneered the ability to directly electrospray and electrospin living cells without compromising their viability. These protocols, now referred to as ''bio-electrosprays'' and ''cell electrospinning'', are rapidly emerging bio-techniques with a plethora of promising applications within the life sciences, in particular to regenerative and therapeutic medicine. Our studies to date, with both bio-electrosprays and cell electrospinning, have demonstrated that a large population of viable cells exist post-treatment, in comparison to controls over both short and long periods as assessed by flow cytometry.Methods: Post-treated mammalian cells are investigated in comparison to controls (culture and needle controls) at a cytogenetic and physiological level. In particular, the study addresses chromosome integrity following these protocols to assess any protocol-inflicted aberrations.Results: The procedures explored failed to inflict any process-driven gross chromosomal aberrations post-treatment.Conclusions: Our preliminary investigations demonstrate no significant compromising affects on the cell''s structure at a cytogenetic or physiological level, post-treatment. Thus, further establishing these protocols as unique direct cell-engineering approaches with a host of biological and medical applications, from the development of tissues to perhaps even organs in the future.