Hemolymph transcriptome analysis of Chinese mitten crab (Eriocheir sinensis) with intact, left cheliped autotomy and bilateral eyestalk ablation. Issue 81 (October 2018)

• Record Type: Journal Article
• Authors: Zhang, Cong; Pang, Yangyang; Zhang, Qian; Huang, Genyong; Xu, Minjie; Tang, Boping; Cheng, Yongxu; Yang, Xiaozhen
• Is Part Of: Fish & shellfish immunology. Issue 81(2018)
• Page Start: 266
• Page End: 275
• Publication Date: 2018-10
• Subjects: Eriocheir sinensis -- Hemolymph -- Autotomy cheliped -- Eyestalk ablation -- Comparative transcriptome; Fishes -- Immunology -- Periodicals; Shellfish -- Immunology -- Periodicals; Poissons -- Immunologie -- Périodiques; Crustacés -- Immunologie -- Périodiques; 571.9617
• DOI: 10.1016/j.fsi.2018.07.025
• Languages: English
• ISSNs: 1050-4648

Abstract

Abstract: In the pond culture of Eriocheir sinensis, high limb-autotomy seriously affects the quality and culture's economic efficiency. Based on our previous studies, limb autotomy can induce the changes of hematological immune response in E. sinensis hemolymph. Eyestalk ablation can accelerate the regeneration of limbs after autotomy. To detect the important functional genes related to the hematological molecular immunity of E. sinensis, we compared and analyzed the hemolymph transcriptome data of the intact crab, left cheliped autotomized crabs and bilateral eyestalk ablation crabs with high-throughput sequencing techniques. The results showed that the three groups obtained 62 172 414, 68 143 682, and 67 811 618 clean reads, respectively. A total of 9567 differentially expressed genes were obtained by multiple comparison of the three groups' libraries. Gene ontology (GO) functional classification analysis shows that the differential genes belong to 42 categories of biological process, cellular components and molecular function. The differentially expressed genes in the three libraries were enriched to 344 specific KEGG metabolic pathways by KEGG enrichment analysis, such as the up-regulated gene (dual oxidase ( Duox ), tyrosine 3-monooxygenase/tryptophan 5-monooxygenase activation protein ( YWHAQ )) in MAPK signaling pathway, the up-regulated gene (aldehyde dehydrogenase 1 ( ALDH 1 )) and down-regulated gene (UDP-glucuronosyltransferase 2 ( UGT 2 )) in metabolism of the xenobiotics by cytochrome P450 pathway, the down-regulated gene (actin gene ( AG ), heat shock protein 90 ( HSP 90 )) in fluid shear stress and atherosclerosis pathway. To verify the expression levels of DEGs identified by RNA-Seq, the above six hematological immune-related genes were selected for qRT-PCR validation, the qRT-PCR results were consistent with the DEGs results. Our research obtained abundant E. sinensis hemolymph transcriptome information by RNA-Seq, which provides multi-level information for the cloning of novel genes and the study of hemolymph molecular immunology mechanisms of E. sinensis . Highlights: The differences between the hemolymph transcriptomes of cheliped autotomized and intact crabs of E. sinensis were analyzed. Hemolymph transcriptome differences between left cheliped autotomized and bilateral eyestalk ablation crabs were analyzed. Autotomized/eyestalk ablation crabs rapidly mobilized immune-related proteins/enzymes to enhance immunity than intact crabs. Autotomized/eyestalk ablation crabs up/down-regulated the expression of related genes to strengthen immunity. As a congenital stress response mechanism, the effect of autotomy on the immune system is less than human-induced injuries.