Identification of a novel C1q complement component in razor clam Sinonovacula constricta and its role in antibacterial activity. Issue 87 (April 2019)

• Record Type: Journal Article
• Authors: Li, Yan; Niu, Donghong; Bai, Yuqi; Lan, Tianyi; Peng, Maoxiao; Dong, Zhiguo; Li, Jiale
• Is Part Of: Fish & shellfish immunology. Issue 87(2019)
• Page Start: 193
• Page End: 201
• Publication Date: 2019-04
• Subjects: Sinonovacula constricta -- Sc-ghC1q -- Bacterial challenge -- Phagocytosis -- Innate immunity -- Antibacterial; Fishes -- Immunology -- Periodicals; Shellfish -- Immunology -- Periodicals; Poissons -- Immunologie -- Périodiques; Crustacés -- Immunologie -- Périodiques; 571.9617
• DOI: 10.1016/j.fsi.2019.01.014
• Languages: English
• ISSNs: 1050-4648

Abstract

Abstract: The serum complement component C1q mediates a variety of immune regulatory functions. Herein, we identified a globular head C1q ( ghC1q ) gene in razor clam Sinonovacula constricta . The complete Sc-ghC1q gene was 872 bp long included an 81 bp 5ʹ-untranslated region (UTR), a 95 bp 3′-UTR with a poly(A) tail, and an open reading frame (ORF) of 696 bp. The mRNA expression of Sc-ghC1q was upregulated in hepatopancreas and hemocytes. After Staphylococcus aureus or Vibrio anguillarum challenge, Sc-ghC1q mRNA transcript abundance was significantly upregulated in hemolymph. Recombinant Sc-ghC1q protein could bind lipopolysaccharide (LPS) and lipoteichoic acid (LTA), and it could agglutinate both Gram-positive and Gram-negative bacteria. Additionally, flow cytometry revealed that Sc-ghC1q strongly promoted phagocytosis in hemocytes. Together, these results demonstrated that Sc-ghC1q played an important role in innate immunity in S. constricta. Highlights: A globular head C1q gene was identified in razor clam Sinonovacula constricta . Sc-ghC1q is upregulated in hemolymph following bacterial pathogen challenge. Sc-ghC1q binds LPS and LTA, and agglutinates Gram-positive and Gram-negative bacteria. Sc-ghC1q strongly promotes phagocytosis in hemocytes.