Kinetics and stereochemistry of LinB-catalyzed δ-HBCD transformation: Comparison of in vitro and in silico results. (September 2018)

• Record Type: Journal Article
• Authors: Heeb, Norbert V.; Mazenauer, Manuel; Wyss, Simon; Geueke, Birgit; Kohler, Hans-Peter E.; Lienemann, Peter
• Is Part Of: Chemosphere. Volume 207(2018)
• Page Start: 118
• Page End: 129
• Publication Date: 2018-09
• Subjects: HBCD biotransformation -- HCH-Converting bacterial enzyme LinB -- Sphingomonadacea -- Michaelis-menten kinetics -- Molecular docking -- Structure prediction; Pollution -- Periodicals; Pollution -- Physiological effect -- Periodicals; Environmental sciences -- Periodicals; Atmospheric chemistry -- Periodicals; 551.511
• DOI: 10.1016/j.chemosphere.2018.05.057
• Languages: English
• ISSNs: 0045-6535

Abstract

Abstract: LinB is a haloalkane dehalogenase found in Sphingobium indicum B90A, an aerobic bacterium isolated from contaminated soils of hexachlorocyclohexane (HCH) dumpsites. We showed that this enzyme also converts hexabromocyclododecanes (HBCDs). Here we give new insights in the kinetics and stereochemistry of the enzymatic transformation of δ-HBCD, which resulted in the formation of two pentabromocyclododecanols (PBCDols) as first- (P1δ, P2δ ) and two tetrabromocyclododecadiols (TBCDdiols) as second-generation products (T1δ, T2δ ). Enzymatic transformations of δ-HBCD, α1 -PBCDol, one of the transformation products, and α2 -PBCDol, its enantiomer, were studied and modeled with Michaelis-Menten (MM) kinetics. Respective MM-parameters K M, v max, k cat / K M indicated that δ-HBCD is the best LinB substrate followed by α2 - and α1 -PBCDol. The stereochemistry of these transformations was modeled in silico, investigating respective enzyme-substrate (ES) and enzyme-product (EP) complexes. One of the four predicted ES-complexes led to the PBCDol product P1δ, identical to α2 -PBCDol with the 1R, 2R, 5S, 6R, 9R, 10S-configuration. An SN 2-like substitution of bromine at C6 of δ-HBCD by Asp-108 of LinB and subsequent hydrolysis of the alkyl-enzyme led to α2 -PBCDol. Modeling results further indicate that backside attacks at C1, C9 and C10 are reasonable too, selectively binding leaving bromide ions in a halide pocket found in LinB. Docking with α2 -PBCDol, also allowed productive enzyme binding. A TBCD-1, 5-diol with the 1S, 2S, 5R, 6R, 9S, 10R-configuration is the predicted second-generation product T1δ . In conclusion, in vitro - and in silico findings now allow a detailed description of step-wise enzymatic dehalohydroxylation reactions of δ-HBCD to specific PBCDols and TBCDdiols at Å-resolution and predictions of their stereochemistry. Graphical abstract: Image 1 Highlights: The dehalogenase LinB from Sphingobium Indicum converts δ-HBCD stereoselectively. Two pentabromocyclododecanols and two tetrabromocyclododecadiols are formed. Transformations of δ-HBCD, α1 -and α2 -PBCDol follow Michaelis-Menten kinetics. Docking experiments revealed four enzyme-substrate complexes allowing SN 2-type reactions. One complex leads to the α2 -PBCDol product with the 1R, 2R, 5S, 6R, 9R, 10S-configuration. Docking experiments predict 1S, 2S, 5R, 6R, 9S, 10R-TBCD-1, 5-diol as a second-generationproduct.