Dried matrix on paper disks: the next generation DBS microsampling technique for managing the hematocrit effect in DBS analysis. (August 2012)

• Record Type: Journal Article
• Authors: Meesters, Roland JW; Zhang, Jamie; van Huizen, Nick A; Hooff, Gero P; Gruters, Rob A; Luider, Theo M
• Is Part Of: Bioanalysis. Volume 4:Number 16(2012)
• Page Start: 2027
• Page End: 2035
• Publication Date: 2012-08
• Subjects: Drugs -- Analysis -- Periodicals; 615.19005
• DOI: 10.4155/bio.12.175
• Languages: English
• ISSNs: 1757-6180

Abstract

Background: The hematocrit effect is a hurdle for successful introduction of the dried blood spot (DBS) in a regulated environment. Recently, attempts were taken to overcome the hematocrit effect by whole-cut DBS analysis. This paper presents the next-generation whole-cut DBS; dried matrix on paper disks (DMPD).Results: DMPD eliminated the hematocrit effect and demonstrated better accuracy and precision than regular DBS with partial punching. Observed accuracy and precision were 6.0 and 2.3% for DMPD, respectively, and -10.4 and 17.1%, for DBS, respectively.Conclusion: The DMPD technique performed better than regular DBS by eliminating the hematocrit effect related blood volume bias. Although this effect was not observed with DMPD, a systematic error of 6.0% was detected and further technical development of DMPD could improve the performance.