Analysis of immunoglobulin and T cell receptor gene expression in ballan wrasse (Labrus bergylta) revealed an extraordinarily high IgM expression in the gut. Issue 87 (April 2019)

• Record Type: Journal Article
• Authors: Bilal, Sumaira; Lie, Kai Kristoffer; Dalum, Alf Seljenes; Karlsen, Odd Andrè; Hordvik, Ivar
• Is Part Of: Fish & shellfish immunology. Issue 87(2019)
• Page Start: 650
• Page End: 658
• Publication Date: 2019-04
• Subjects: IgM -- TCRδ -- Intestine -- Stomach-less -- Protein A -- Mucus -- Natural antibodies; Fishes -- Immunology -- Periodicals; Shellfish -- Immunology -- Periodicals; Poissons -- Immunologie -- Périodiques; Crustacés -- Immunologie -- Périodiques; 571.9617
• DOI: 10.1016/j.fsi.2019.02.007
• Languages: English
• ISSNs: 1050-4648

Abstract

Abstract: The serum IgM concentration of ballan wrasse is relatively high, estimated to approximately 13 mg/ml in adult wild fish of 800 g. The present study revealed an unusual high abundance of IgM mRNA in the gut of ballan wrasse. Initially, transcripts encoding IgM, IgT, IgD, TCRα, TCRδ and CD3ε were quantified by RT-qPCR in several tissues of wild caught fish (approx. 800 g), indicating an elevated immune activity in hindgut and an extraordinarily high expression of IgM. Subsequently, a new RT-qPCR analysis was performed on the entire intestine, cut into four different segments, of reared fish (32–100 g). The analysis indicated immune activity along the entire intestine, but not as strong as in the hindgut. Furthermore, similar to the larger fish, the relative abundance of IgM transcripts was higher in the hindgut than in kidney and spleen, although the absolute level of IgM was in general higher in the larger fish. The secreted form of IgM was completely dominant in comparison to the membrane bound form of IgM and the other analysed genes. IgM was purified from gut mucus and external mucosal surfaces by magnetic beads coated with protein A. Mucus IgM reacted with rabbit antisera raised against serum IgM and contained subunits of the same size. Regarding the elevated immune activity in the intestine it is tempting to speculate on a possible compensatory strategy in this lineage of stomach-less fish, and that natural antibodies have an important role in the first line defence. Graphical abstract: Image 1 Highlights: Ballan wrasse shows an unusually high IgM gene expression in gut. Mucus IgM can be purified with magnetic beads coated with protein A. The expression pattern of TCRδ indicates an important role in mucosal immunity.