Anti-inflammatory effect of a novel synthetic compound 1-((4-fluorophenyl)thio)isoquinoline in RAW264.7 macrophages and a zebrafish model. Issue 87 (April 2019)

• Record Type: Journal Article
• Authors: Kim, Geum Ran; Yang, Jung Yoon; Hwang, Kyu-Seok; Kim, Seong Soon; Chae, Jin Sil; Kan, Hyemin; Ahn, Jin Hee; Lee, Won Mi; Ahn, Se Hwan; Lee, Yong-Moon; Bae, Myung Ae; Shin, Dae-Seop
• Is Part Of: Fish & shellfish immunology. Issue 87(2019)
• Page Start: 395
• Page End: 400
• Publication Date: 2019-04
• Subjects: Zebrafish -- Anti-Inflammation -- RAW264.7 -- Neutrophil; Fishes -- Immunology -- Periodicals; Shellfish -- Immunology -- Periodicals; Poissons -- Immunologie -- Périodiques; Crustacés -- Immunologie -- Périodiques; 571.9617
• DOI: 10.1016/j.fsi.2019.01.030
• Languages: English
• ISSNs: 1050-4648

Abstract

Abstract: The compound, 1-((4-fluorophenyl)thio)isoquinoline (FPTQ), is a synthetic isoquinoline derivative. To test the anti-inflammatory effect of FPTQ, we used neutrophil-specific transgenic zebrafish Tg(mpx :: EGFP) i114 line and lipopolysaccharide (LPS)-stimulated RAW264.7 cells. We also used two different methods, involving tail transection and LPS stimulation in the zebrafish model. Neutrophils translocation in the zebrafish tail-transected model was inhibited by FPTQ. Neutrophil aggregation was also inhibited by FPTQ in the LPS-stimulated zebrafish model. Decreased mRNA expression of the pro-inflammatory cytokine genes, interleukin-1β ( il-1β ) and interleukin-6 (il-6), was found in zebrafish larvae injected with FPTQ. Additionally, production of nitric oxide was inhibited by FPTQ in RAW264.7 macrophage cells treated with LPS. Moreover, the mRNA expression of Il-1β and Il-6 suppressed by FPTQ treatment in RAW264.7 macrophage cells, and an enzyme immunoassay showed that FPTQ suppressed the secretion of IL-1β and IL-6 in RAW264.7 cells. These results demonstrate that FPTQ reduced inflammatory responses and, therefore, suggest that it may be effective as an anti-inflammatory agent. Highlights: FPTQ significantly inhibited inflammatory responses in zebrafish larvae. FPTQ inhibited translocation of neutrophils to the inflammatory site. FPTQ suppressed the inflammatory cytokines in zebrafish and RAW264.7 cells. The concentration of FPTQ in zebrafish larvae was quantified by LC-MS/MS.