The Impact of Perineuronal Net Digestion Using Chondroitinase ABC on the Intrinsic Physiology of Cortical Neurons. (15th September 2018)

• Record Type: Journal Article
• Authors: Chu, Philip; Abraham, Reena; Budhu, Kumarie; Khan, Usma; De Marco Garcia, Natalia; Brumberg, Joshua C.
• Is Part Of: Neuroscience. Volume 388(2018)
• Page Start: 23
• Page End: 35
• Publication Date: 2018-09-15
• Subjects: AHP after-hyperpolarization -- AP action potential -- ECM Extracellular Matrix -- FS Fast Spiking -- PBS phosphate-buffered saline -- PFA paraformaldehyde -- PMBSF posterior medial barrel subfield -- PNNs perineuronal nets -- RMP resting membrane potentials -- sACSF sucrose artificial cerebral spinal fluid -- WFA Wisteria Floribunda Agglutinin; barrel cortex -- inhibitory interneurons -- perineuronal nets -- intrinsic physiology; Neurochemistry -- Periodicals; Neurophysiology -- Periodicals; Neurology -- Periodicals; Neurochimie -- Périodiques; Neurophysiologie -- Périodiques; Neurochemistry; Neurophysiology; Electronic journals; Periodicals; Electronic journals; 612.8
• DOI: 10.1016/j.neuroscience.2018.07.004
• Languages: English
• ISSNs: 0306-4522

Abstract

Highlights: Digestion of PNNs affects passive & active properties of fast spiking interneurons. Digestion of PNNs affects synaptic transmission of inhibitory interneurons. Digestion of PNNs does not significantly impact excitatory neurons. Abstract: Perineuronal nets (PNNs) are a form of aggregate Extracellular Matrix (ECM) in the brain. Recent evidence suggests that the postnatal deposition of PNNs may play an active role in regulating neuroplasticity and, potentially, neurological disorders. Observations of high levels of PNN expression around somas, proximal dendrites, and axon initial segments of a subtype of neurons have also led to proposals that PNNs may modulate the intrinsic properties of the neurons they ensheathe. While high levels of PNNs are postnatally expressed throughout the neocortex, it is still unclear how they impact the neuronal physiology of the many classes and subtypes of neurons that exist. In this study, we demonstrate that Chondroitinase ABC digestion of PNNs from acute cortical slices from juvenile mice (P28-35) resulted in neuron-specific impacts on intrinsic physiology. Fast spiking (FS) interneurons showed decreased input resistance, resting membrane potential (RMP), reduced action potential (AP) peaks and altered spontaneous synaptic inputs. Low-Threshold Spiking interneurons showed altered rebound depolarizations and decreased frequency of spontaneous synaptic inputs. Putative excitatory neurons; regular spiking, bursting, and doublet phenotypes did not demonstrate any alterations. Our data indicate that chABC-sensitive PNNs may specifically regulate the intrinsic and synaptic physiology of inhibitory interneurons.