Current methods for global proteome identification. (October 2012)

• Record Type: Journal Article
• Authors: Vaudel, Marc; Sickmann, Albert; Martens, Lennart
• Is Part Of: Expert review of proteomics. Volume 9:Number 5(2012)
• Page Start: 519
• Page End: 532
• Publication Date: 2012-10
• Subjects: bioinformatics -- identification -- proteomics; Proteins -- Biotechnology -- Periodicals; Proteomics -- Periodicals; 572.6
• DOI: 10.1586/epr.12.51
• Languages: English
• ISSNs: 1478-9450

Abstract

In a time frame of a few decades, protein identification went from laborious single protein identification to automated identification of entire proteomes. This shift was enabled by the emergence of peptide-centric, gel-free analyses, in particular the so-called shotgun approaches, which not only rely on extensive experiments, but also on cutting-edge data processing methods. The present review therefore provides an overview of a shotgun proteomics identification workflow, listing the state-of-the-art methods involved and software that implement these. The authors focus on freely available tools where possible. Finally, data analysis in the context of emerging across-omics studies will also be discussed briefly, where proteomics goes beyond merely delivering a list of protein accession numbers.