Insights into the virulence of oral biofilms: discoveries from proteomics. (June 2012)

• Record Type: Journal Article
• Authors: Kuboniwa, Masae; Tribble, Gena D; Hendrickson, Erik L; Amano, Atsuo; Lamont, Richard J; Hackett, Murray
• Is Part Of: Expert review of proteomics. Volume 9:Number 3(2012)
• Page Start: 311
• Page End: 323
• Publication Date: 2012-06
• Subjects: biofilm -- dental plaque -- Fusobacterium nucleatum -- host response -- microbial proteomics -- multidimensional protein identification technology -- Porphyromonas gingivalis -- Prevotella intermedia -- quantitative proteomics -- Treponema denticola; Proteins -- Biotechnology -- Periodicals; Proteomics -- Periodicals; 572.6
• DOI: 10.1586/epr.12.16
• Languages: English
• ISSNs: 1478-9450

Abstract

This review covers developments in the study of polymicrobial communities, biofilms and selected areas of host response relevant to dental plaque and related areas of oral biology. The emphasis is on recent studies in which proteomic methods, particularly those using mass spectrometry as a readout, have played a major role in the investigation. The last 5–10 years have seen a transition of such methods from the periphery of oral biology to the mainstream, as in other areas of biomedical science. For reasons of focus and space, the authors do not discuss biomarker studies relevant to improved diagnostics for oral health, as this literature is rather substantial in its own right and deserves a separate treatment. Here, global gene regulation studies of plaque-component organisms, biofilm formation, multispecies interactions and host–microbe interactions are discussed. Several aspects of proteomics methodology that are relevant to the studies of multispecies systems are commented upon.