Glycopeptide microarray for autoantibody detection in cancer. (August 2011)

• Record Type: Journal Article
• Authors: Reis, Celso A; Campos, Diana; Osóório, Hugo; Santos, Lúúcio Lara
• Is Part Of: Expert review of proteomics. Volume 8:Number 4(2011)
• Page Start: 435
• Page End: 437
• Publication Date: 2011-08
• Subjects: autoantibodies -- cancer biomarkers -- cancer detection -- colon -- glycopeptides -- microarray -- rectum; Proteins -- Biotechnology -- Periodicals; Proteomics -- Periodicals; 572.6
• DOI: 10.1586/epr.11.30
• Languages: English
• ISSNs: 1478-9450

Abstract

Evaluation of: Pedersen JW, Blixt O, Bennett EP et al. Seromic profiling of colorectal cancer patients with novel glycopeptide microarray. Int. J. Cancer 128(8), 1860––1871 (2011). Autoantibodies to cancer-associated antigens hold promise as sensitive biomarkers for cancer detection. Based on this hypothesis, and knowing that O -glycans on proteins constitute a source of possible epitopes recognized by autoantibodies, Pedersen and colleagues have generated a glycopeptide array displaying a comprehensive library of glycopeptides and glycoproteins derived from human mucins. The profiling of sera immunoreactivity of colon cancer patients allowed the identification of cancer-associated autoantibodies to various mucin (MUC)1 and MUC4 glycopeptides carrying aberrant glycosylation. This article provides evidence for the value of glycopeptides displaying cancer-associated glycans in diagnostic applications, and opens new avenues for the expansion to other protein glycoforms, as well as to further applications of such a microarray strategy for other post-translational modifications of proteins in the search for cancer biomarker.