Rapid Extracellular Matrix Remodeling via Gene‐Electrospun Fibers as a "Patch" for Tissue Regeneration. (16th February 2021)

• Record Type: Journal Article
• Authors: Jin, Jing; Saiding, Qimanguli; Wang, Xianjing; Qin, Menglu; Xiang, Yi; Cheng, Ruoyu; Cui, Wenguo; Chen, Xinliang
• Is Part Of: Advanced functional materials. Volume 31:Number 15(2021)
• Page Start: n/a
• Page End: n/a
• Publication Date: 2021-02-16
• Subjects: core–shell structures -- drug delivery -- electrospun fibers -- extracellular matrix -- tissue regeneration; Materials -- Periodicals; Chemical vapor deposition -- Periodicals; 620.11
• DOI: 10.1002/adfm.202009879
• Languages: English
• ISSNs: 1616-301X

Abstract

Abstract: A break of extracellular matrix (ECM) balance can cause several degenerative changes in soft tissues. To reverse the imbalance fundamentally, a recently developed treatment, gene therapy, came to attention. However, the efficiency of the approach is limited because long‐term localized presence and bioactivity are difficult to achieve. Here, reconstituted lysyl oxidase‐like 1 (LOXL1) plasmids (pLOXL1) are loaded into nanoliposomes by a microfluidic chip, followed by encapsulation into the core layer of core–shell nanofibers by microsol‐electrospinning to achieve local accumulation and biological availability of the constructs and enable rapid ECM response. Results show that the pLOXL1‐Lipo@PLCL‐HA achieves the sustained release of pLOXL1 over a 30‐day period, with its transfection efficiency maintained above 50%. In a rabbit model of abdominal hernia, the long‐term collagen remodeling density is raised by over 90% in the pLOXL1‐Lipo@PLCL‐HA implanted animals compared to the control animals. The expression levels of ECM gene (COL1A1, COL3A1, Elastin, Fubilin5) are significantly increased. Collectively, this study establishes that pLOXL1‐Lipo@PLCL‐HA accelerates local ECM reconstruction via effective and reliable gene delivery as a potential base material of a "patch" for pelvic floor repairment, and identifies the key principles for design of LOXL1‐incorporated scaffolds for ECM regeneration. Abstract : A composite system of lysyl oxidase and scaffold materials is successfully constructed for rapid extracellular matrix remodeling via gene‐electrospun fibers as a "patch." It can adjust the balance between LOXL1‐mediated extracellular matrix (ECM) deposition and degradation, thus promoting partial restoration and reconstruction of support strength quickly. This gene‐electrospun fiber works like a "patch", while the weakness of the ECM emerges as a "bug".