Multitarget fluorescence in situ hybridization diagnostic applications in solid and hematological tumors. (1st February 2021)

• Record Type: Journal Article
• Authors: Zito Marino, Federica; Brunelli, Matteo; Rossi, Giulio; Calabrese, Giuseppe; Caliò, Anna; Nardiello, Pamela; Martignoni, Guido; Squire, Jeremy A.; Cheng, Liang; Massi, Daniela; Franco, Renato
• Is Part Of: Expert review of molecular diagnostics. Volume 21:Number 2(2021)
• Page Start: 161
• Page End: 173
• Publication Date: 2021-02-01
• Subjects: Multitarget fluorescence in situ hybridization -- non-small cell lung cancer -- melanoma -- renal cell neoplasia -- bladder carcinoma -- multiple myeloma; Molecular diagnosis -- Periodicals; 616.0758205
• DOI: 10.1080/14737159.2021.1887733
• Languages: English
• ISSNs: 1473-7159

Abstract

ABSTRACT: Introduction : Multitarget FISH (mFISH) is a technique allowing for simultaneous detection of multiple targets sequences on the same slide through the choice of spectrally distinct fluorophore labels. The mFISH could represent a useful tool in the field of precision oncology. Areas covered : This review discusses the potential applications of mFISH technology in the molecular diagnosis of different solid and hematological tumors, including non-small cell lung cancers, melanomas, renal cell carcinomas, bladder carcinomas, germ cell tumors, and multiple myeloma, as commonly required in the clinical practice. Expert Opinion : In this emerging era of the tailored therapies and newer histo-molecular classifications, there are increasing numbers of predictive and diagnostic biomarkers required for effective clinical care. The mFISH approach may have several applications in the common clinical practice, improving the molecular diagnosis in terms of time, cost and preservation of biomaterial for tumors with a limited amount of tumor available. The mFISH provides several advantages compared to other high-throughput technologies; however, it requires high level of expertise required to interpret complex results.