A fluorometric and colorimetric dual-signal nanoplatform for ultrasensitive visual monitoring of the activity of alkaline phosphatase. Issue 13 (26th February 2021)

• Record Type: Journal Article
• Authors: An, Jia; Hu, Yongqin; Liu, Guoyi; Chen, Meizhu; Chen, Rubing; Lyu, Ying; Yuan, Mengdi; Luo, Mengfei; Liu, Yufei
• Is Part Of: Journal of materials chemistry. Volume 9:Issue 13(2021)
• Page Start: 2998
• Page End: 3004
• Publication Date: 2021-02-26
• Subjects: Materials -- Periodicals; Chemistry, Analytic -- Periodicals; Biomedical materials -- Research -- Periodicals; 543.0284
• DOI: 10.1039/d0tb02531c
• Languages: English
• ISSNs: 2050-750X

Abstract

Abstract : Considering the limited sensitivity and accuracy of single-signal assay strategies, the multi-signal assay strategy has sparked significant excitement in recent years. Abstract : Considering the limited sensitivity and accuracy of single-signal assay strategies, the multi-signal assay strategy has sparked significant excitement in recent years. In this study, for the first time, we reported a one-pot method in situ synthesis of carbon-containing nanoparticles (CNPs) via p -aminophenol (AP) and diethylenetriamine (DETA). The CNP solution exhibits yellow and light blue fluorescence under UV-light. Moreover, the CNPs exhibited excellent photoluminescence stability even under extreme conditions. Inspired by the alkaline phosphatase (ALP)-triggered specific catalytic reaction, we constructed an ultrasensitive fluorescence and colorimetric two-channel strategy for monitoring the ALP activity. By optimizing the detection parameters, the detection limits for both fluorometric and colorimetric were 0.05 mU mL −1 . Moreover, the strategy showed high specificity and was successfully applied to monitor the ALP activity level in human serum samples. The analytical strategy opened a new window for the detection of the ALP activity, screening of the ALP inhibitor, and disease diagnosis.