Bioinformatical dissection of fission yeast DNA replication origins. Issue 7 (22nd July 2020)

• Record Type: Journal Article
• Authors: Masuda, Koji; Renard-Guillet, Claire; Shirahige, Katsuhiko; Sutani, Takashi
• Is Part Of: Open biology. Volume 10:Issue 7(2020)
• Publication Date: 2020-07-22
• Subjects: replication origins -- pre-replication complex -- fission yeast -- ChIP-seq -- machine learning; Biology -- Periodicals; 570
• DOI: 10.1098/rsob.200052
• Languages: English
• ISSNs: 2046-2441

Abstract

Abstract : Abstract : Replication origins in eukaryotes form a base for assembly of the pre-replication complex (pre-RC), thereby serving as an initiation site of DNA replication. Characteristics of replication origin vary among species. In fission yeast Schizosaccharomyces pombe, DNA of high AT content is a distinct feature of replication origins; however, it remains to be understood what the general molecular architecture of fission yeast origin is. Here, we performed ChIP-seq mapping of Orc4 and Mcm2, two representative components of the pre-RC, and described the characteristics of their binding sites. The analysis revealed that fission yeast efficient origins are associated with two similar but independent features: a ≥15 bp-long motif with stretches of As and an AT-rich region of a few hundred bp. The A-rich motif was correlated with chromosomal binding of Orc, a DNA-binding component in the pre-RC, whereas the AT-rich region was associated with efficient binding of the DNA replicative helicase Mcm. These two features, in combination with the third feature, a transcription-poor region of approximately 1 kb, enabled to distinguish efficient replication origins from the rest of chromosome arms with high accuracy. This study, hence, provides a model that describes how multiple functional elements specify DNA replication origins in fission yeast genome.