SARS-CoV-2 envelope protein topology in eukaryotic membranes. Issue 9 (9th September 2020)

• Record Type: Journal Article
• Authors: Duart, Gerard; García-Murria, Mª Jesús; Grau, Brayan; Acosta-Cáceres, José M.; Martínez-Gil, Luis; Mingarro, Ismael
• Is Part Of: Open biology. Volume 10:Issue 9(2020)
• Publication Date: 2020-09-09
• Subjects: coronavirus -- envelope protein -- membrane insertion -- SARS-CoV-2 -- topology; Biology -- Periodicals; 570
• DOI: 10.1098/rsob.200209
• Languages: English
• ISSNs: 2046-2441

Abstract

Abstract : Abstract : Coronavirus E protein is a small membrane protein found in the virus envelope. Different coronavirus E proteins share striking biochemical and functional similarities, but sequence conservation is limited. In this report, we studied the E protein topology from the new SARS-CoV-2 virus both in microsomal membranes and in mammalian cells. Experimental data reveal that E protein is a single-spanning membrane protein with the N-terminus being translocated across the membrane, while the C-terminus is exposed to the cytoplasmic side (Ntlum /Ctcyt ). The defined membrane protein topology of SARS-CoV-2 E protein may provide a useful framework to understand its interaction with other viral and host components and contribute to establish the basis to tackle the pathogenesis of SARS-CoV-2.