Cancer biomarker profiling using nanozyme containing iron oxide loaded with gold particles. Issue 167 (24th June 2020)

• Record Type: Journal Article
• Authors: Akpe, Victor; Shiddiky, Muhammad J. A.; Kim, Tak H.; Brown, Christopher L.; Yamauchi, Yusuke; Cock, Ian E.
• Is Part Of: Journal of the Royal Society interface. Volume 17:Issue 167(2020)
• Publication Date: 2020-06-24
• Subjects: amperometry detection -- cancer biomarkers -- limit of detection -- biosensor amplification; Physical sciences -- Research -- Periodicals; Life sciences -- Research -- Periodicals; Interdisciplinary research -- Periodicals; 570.5
• DOI: 10.1098/rsif.2020.0180
• Languages: English
• ISSNs: 1742-5689

Abstract

Abstract : Nanozymes are nanomaterials with intrinsic magnetism and superparamagnetic properties. In the presence of an external magnet, nanozyme particles aggregate and redisperse without a foreign attraction. We evaluated the performances of nanozyme by changing the biosensing platforms and substituting other biological variants for a complete cancer assay detection. We investigated the expression of morphological variants in the transmission of signals using an electrochemical method. The signal responses, including signal enhancement with the nanozyme (Au-Fe2 O3 ), showed a wide capturing range (greater than 80%, from 10 2 to 10 5 cells ml −1 in phosphate-buffered saline buffer, pH 7.4). The platform showed a fast response time within a dynamic range of 10–10 5 cells ml −1 for the investigated T47D cancer cell line. We also obtained higher responses for anti-HER2 (human epidermal receptor 2)/streptavidin interface as the biosensing electrode in the presence of T47D cancer cells. The positive assay produced a sixfold increase in current output compared to the negative target or negative biological variant. We calculated the limit of detection at 0.4 U ml −1, and of quantitation at 4 U ml −1 (units per millilitre). However, blood volume amounts in clinical settings may constrain diagnosis and increase detection limit value significantly.