InsP6-Sensitive Variants of the Gle1 mRNA Export Factor Rescue Growth and Fertility Defects of the ipk1 Low-Phytic-Acid Mutation in Arabidopsis. Issue 2 (10th February 2015)

• Record Type: Journal Article
• Authors: Lee, Ho-Seok; Lee, Du-Hwa; Cho, Hui Kyung; Kim, Song Hee; Auh, Joong Hyuck; Pai, Hyun-Sook
• Is Part Of: The Plant Cell. Volume 27:Issue 2(2015)
• Page Start: 417
• Page End: 431
• Publication Date: 2015-02-10
• DOI: 10.1105/tpc.114.132134
• Languages: English
• ISSNs: 1040-4651

Abstract

Abstract : Expression of Gle1 variants with modified InsP6 binding pockets rescues mRNA export defects of the ipk1 InsP6 -deficient mutant, suggesting a critical role for Gle1 in mediating InsP6 function. Abstract: Myo -inositol-1, 2, 3, 4, 5, 6-hexa kis phosphate (InsP6 ), also known as phytic acid, accumulates in large quantities in plant seeds, serving as a phosphorus reservoir, but is an animal antinutrient and an important source of water pollution. Here, we report that Gle1 (GLFG lethal 1) in conjunction with InsP6 functions as an activator of the ATPase/RNA helicase LOS4 (low expression of osmotically responsive genes 4), which is involved in mRNA export in plants, supporting the Gle1-InsP6 -Dbp5 (LOS4 homolog) paradigm proposed in yeast. Interestingly, plant Gle1 proteins have modifications in several key residues of the InsP6 binding pocket, which reduce the basicity of the surface charge. Arabidopsis thaliana Gle1 variants containing mutations that increase the basic charge of the InsP6 binding surface show increased sensitivity to InsP6 concentrations for the stimulation of LOS4 ATPase activity in vitro. Expression of the Gle1 variants with enhanced InsP6 sensitivity rescues the mRNA export defect of the ipk1 ( inositol 1, 3, 4, 5, 6-penta kis phosphate 2-kinase ) InsP6 -deficient mutant and, furthermore, significantly improves vegetative growth, seed yield, and seed performance of the mutant. These results suggest that Gle1 is an important factor responsible for mediating InsP6 functions in plant growth and reproduction and that Gle1 variants with increased InsP6 sensitivity may be useful for engineering high-yielding low-phytate crops.