IDN2 Interacts with RPA and Facilitates DNA Double-Strand Break Repair by Homologous Recombination in Arabidopsis. Issue 3 (21st February 2017)

• Record Type: Journal Article
• Authors: Liu, Mingming; Ba, Zhaoqing; Costa-Nunes, Pedro; Wei, Wei; Li, Lanxia; Kong, Fansi; Li, Yan; Chai, Jijie; Pontes, Olga; Qi, Yijun
• Is Part Of: The Plant Cell. Volume 29:Issue 3(2017)
• Page Start: 589
• Page End: 599
• Publication Date: 2017-02-21
• DOI: 10.1105/tpc.16.00769
• Languages: English
• ISSNs: 1040-4651

Abstract

Abstract : IDN2, a dsRNA binding protein, interacts with replication protein A and facilitates its release from ssDNA tails and the subsequent recruitment of RAD51 at DSB sites, promoting homologous recombination repair. Abstract: Repair of DNA double-strand breaks (DSBs) is critical for the maintenance of genome integrity. We previously showed that DSB -induced small RNAs (diRNAs) facilitate homologous recombination-mediated DSB repair in Arabidopsis thaliana . Here, we show that INVOLVED IN DE NOVO2 (IDN2), a double-stranded RNA binding protein involved in small RNA-directed DNA methylation, is required for DSB repair in Arabidopsis. We find that IDN2 interacts with the heterotrimeric replication protein A (RPA) complex. Depletion of IDN2 or the diRNA binding ARGONAUTE2 leads to increased accumulation of RPA at DSB sites and mislocalization of the recombination factor RAD51. These findings support a model in which IDN2 interacts with RPA and facilitates the release of RPA from single-stranded DNA tails and subsequent recruitment of RAD51 at DSB sites to promote DSB repair.