Nod Factor Receptors Form Heteromeric Complexes and Are Essential for Intracellular Infection in Medicago Nodules. Issue 10 (28th October 2014)

• Record Type: Journal Article
• Authors: Moling, Sjef; Pietraszewska-Bogiel, Anna; Postma, Marten; Fedorova, Elena; Hink, Mark A.; Limpens, Erik; Gadella, Theodorus W.J.; Bisseling, Ton
• Is Part Of: The Plant Cell. Volume 26:Issue 10(2014)
• Page Start: 4188
• Page End: 4199
• Publication Date: 2014-10-28
• DOI: 10.1105/tpc.114.129502
• Languages: English
• ISSNs: 1040-4651

Abstract

Abstract : The Medicago truncatula Nod factor receptors LYK3 and NFP accumulate in a narrow zone of approximately two cell layers in the nodule apex, where they form heteromeric complexes. This accumulation at the border of the meristem and the infection zone controls bacterial release, and its spatial restriction most likely prevents defense-like responses. Abstract: Rhizobial Nod factors are the key signaling molecules in the legume-rhizobium nodule symbiosis. In this study, the role of the Nod factor receptors NOD FACTOR PERCEPTION (NFP) and LYSIN MOTIF RECEPTOR-LIKE KINASE3 (LYK3) in establishing the symbiotic interface in root nodules was investigated. It was found that inside Medicago truncatula nodules, NFP and LYK3 localize at the cell periphery in a narrow zone of about two cell layers at the nodule apex. This restricted accumulation is narrower than the region of promoter activity/mRNA accumulation and might serve to prevent the induction of defense-like responses and/or to restrict the rhizobium release to precise cell layers. The distal cell layer where the receptors accumulate at the cell periphery is part of the meristem, and the proximal layer is part of the infection zone. In these layers, the receptors can most likely perceive the bacterial Nod factors to regulate the formation of symbiotic interface. Furthermore, our Förster resonance energy transfer-fluorescence lifetime imaging microscopy analysis indicates that NFP and LYK3 form heteromeric complexes at the cell periphery in M. truncatula nodules.