A review of solvent freeze-out technology for protein crystallization. Issue 14 (18th March 2021)

• Record Type: Journal Article
• Authors: Ming, Hui; Zhu, Ming-Fu; Li, Lu; Liu, Qing-Bin; Yu, Wen-Hua; Wu, Zi-Qing; Liu, Yong-Ming
• Is Part Of: CrystEngComm. Volume 23:Issue 14(2021)
• Page Start: 2723
• Page End: 2732
• Publication Date: 2021-03-18
• Subjects: Crystals -- Periodicals; Crystal growth -- Periodicals; Crystallography -- Periodicals; Cristaux -- Périodiques; Cristaux -- Croissance -- Périodiques; Cristallographie -- Périodiques; 548
• DOI: 10.1039/d1ce00005e
• Languages: English
• ISSNs: 1466-8033

Abstract

Abstract : In this review, we summarize important advances in solvent freeze-out (SFO) technology for protein crystallization, including the background of SFO, its fundamental principle, and some crucial conditions and factors for optimizing SFO technology. Abstract : The demand for proteins with high purity, high activity and high stability is increasing, especially in the biopharmaceutical industry. Traditional industrial protein production technologies include chromatography, which is expensive, and salting out and traditional crystallization methods, which require large amounts of salt and have an adverse effect on the environment. In recent years, a new method—solvent freeze-out (SFO) technology for protein crystallization—has been developed. Compared with traditional methods, SFO requires less salt, and can be carried out at low temperatures, resulting in more stable and active proteins. In addition, SFO can control the nucleation and crystal growth process under different supersaturation levels by setting appropriate operating temperatures, which can ensure industrial application of this technology. This paper reviews important advances in SFO technology for the crystallization of proteins.