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Capturing protein droplets: label-free visualization and detection of protein liquid–liquid phase separation with an aggregation-induced emission fluorogen. Issue 31 (19th March 2021)
Record Type:
Journal Article
Title:
Capturing protein droplets: label-free visualization and detection of protein liquid–liquid phase separation with an aggregation-induced emission fluorogen. Issue 31 (19th March 2021)
Main Title:
Capturing protein droplets: label-free visualization and detection of protein liquid–liquid phase separation with an aggregation-induced emission fluorogen
Abstract : Methods for visualizing label-free protein liquid–liquid phase separation and measuring the viscosity of the protein condensed phase are developed. Abstract : We developed a new method for protein droplet visualization by means of a droplet probe (DroProbe) based on an aggregation-induced emission (AIE) fluorogen. A simple method for viscosity comparison of the protein condensed phase based on the lifetime of the DroProbe was also developed.