Bio-CaRGOS: capture and release gels for optimized storage of hemoglobin. Issue 22 (7th April 2021)

• Record Type: Journal Article
• Authors: Boylan, Jack; Chauhan, Rajat; Koneru, Kavya; Bansal, Meenakshi; Kalbfleisch, Theodore; Potnis, Chinmay S.; Hartline, Kendall; Keynton, Robert S.; Gupta, Gautam
• Is Part Of: RSC advances. Volume 11:Issue 22(2021)
• Page Start: 13034
• Page End: 13039
• Publication Date: 2021-04-07
• Subjects: Chemistry -- Periodicals; 540.5
• DOI: 10.1039/d1ra00987g
• Languages: English
• ISSNs: 2046-2069

Abstract

Abstract : A schematic of encapsulation of hemoglobin within Bio:CaRGOS formulations is summarized in the graphic, demonstrating sol–gel encapsulation as a method to stabilize hemoglobin, followed by an efficient hemoglobin release using polyethylene glycol (PEG). Abstract : Room temperature biospecimen storage for prolonged periods is essential to eliminate energy consumption by ultra-low freezing or refrigeration-based storage techniques. State of the art practices that sufficiently minimize the direct or hidden costs associated with cold-chain logistics include ambient temperature storage of biospecimens ( i.e., DNA, RNA, proteins, lipids) in the dry state. However, the biospecimens are still well-exposed to the stress associated with drying and reconstitution cycles, which augments the pre-analytical degradation of biospecimens prior to their downstream processing. An aqueous storage solution that can eliminate these stresses which are correlated to several cycles of drying/rehydration or freezing of biospecimens, is yet to be achieved by any current technology. In our study, we have addressed this room temperature biospecimen-protection challenge using aqueous capture and release gels for optimized storage (Bio-CaRGOS) of biospecimens. Herein, we have demonstrated a single-step ∼95% recovery of a metalloprotein hemoglobin at room temperature using a cost-effective standard microwave-based aqueous formulation of Bio-CaRGOS. Although hemoglobin samples are currently stored at sub-zero or under refrigeration (4 °C) conditions to avoid loss of integrity and an unpredictable diagnosis during their downstream assays, our results have displayed an unprecedented room temperature integrity preservation of hemoglobin. Bio-CaRGOS formulations efficiently preserve hemoglobin in its native state, with single-step protein recovery of ∼95% at ambient conditions (1 month) and ∼96% (7 months) under refrigeration conditions. In contrast, two-thirds of the control samples degrade under ambient (1 month) and refrigeration (7 months) settings.