Effect of skimmed milk on intestinal tract: Prevention of increased reactive oxygen species and nitric oxide formation. (July 2021)

• Record Type: Journal Article
• Authors: Pinho, Susana C.M.; Faria, Miguel A.; Melo, Armindo; Pinto, Edgar; Almeida, Agostinho; Alves, Rui; Cabrita, Ana R.J.; Fonseca, António J.M.; Ferreira, Isabel M.P.L.V.O.
• Is Part Of: International dairy journal. Volume 118(2021)
• Publication Date: 2021-07
• Subjects: Dairying -- Periodicals; Industrie laitière -- Périodiques; Electronic journals; 338.1762142
• DOI: 10.1016/j.idairyj.2021.105046
• Languages: English
• ISSNs: 0958-6946

Abstract

Abstract: The capacity of skimmed milk to neutralise increased reactive oxygen species (ROS) and to attenuate nitric oxide (NO) production, as well as to present cytoprotective effect at the intestinal level was assessed after in vitro gastro-intestinal digestion. The impact on ROS modulation was evaluated at a non-cytotoxic concentration of hydrogen peroxide (H2 O2 ) in a co-culture of Caco-2 and HT-29 intestinal cells. In parallel, a cytotoxic concentration of H2 O2 was used to study the effect of digested milk against induced cell apoptosis. Concerning induced NO production, it was evaluated using the model lipopolysaccharide (LPS)-stimulated RAW264.7 macrophage cells. Results showed that digested milk prevented the increase of basal ROS level in the intestinal epithelium and attenuated NO production by LPS-stimulated macrophage cells. In the H2 O2 -induced cytotoxicity assay, digested milk had no protection against apoptosis, confirmed by the failure in attenuating activated caspase-3/7.